Test Code TIBC Total Iron Binding Capacity, Serum
Specimen Required
Only orderable as part of profile. For more information see SFEC / Iron and Total Iron Binding Capacity, Serum
Patient Preparation:
1. Fasting (12 hours)
2. For 24 hours before collection, patient should not take iron-containing supplements.
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Draw blood before noon (preferred).
2. Within 2 hours of collection, serum gel tubes should be centrifuged.
3. Within 2 hours of collection, red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial.
Secondary ID
2501Useful For
Screening for chronic iron overload diseases, particularly hereditary hemochromatosis
Method Name
Only orderable as part of profile. For more information see SFEC / Iron and Total Iron Binding Capacity, Serum
Turbidity/Calculation
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 21 days | |
Frozen | 365 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
Only orderable as part of profile. For more information see SFEC / Iron and Total Iron-Binding Capacity, Serum.
250-400 mcg/dL
Interpretation
Total iron-binding capacity concentrations are elevated in anemia of chronic disease and iron overload conditions.(1)
Total iron-binding capacity concentrations are decreased in iron deficiency, iron deficiency anemia, and iron-refractory iron deficiency anemia.(1)
Method Description
Transferrin:
Anti-transferrin antibodies react with the antigen in the sample to form an antigen/antibody complex. Following agglutination, this is measured turbidimetrically. Addition of polyethylene glycol allows the reaction to progress rapidly to the end point and increases sensitivity. The result is used to calculate the total iron-binding capacity and is not included on the report.(Package insert: TRSF2. Roche Diagnostics; v10.0, 04/2022)
Total Iron-Binding Capacity:
This test calculates the total iron-binding capacity (TIBC) using results obtained from transferrin analysis. The following calculation is performed in the laboratory information system.
TIBC = Transferrin x 1.18
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 2 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
83550
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TIBC | Total Iron Binding Capacity | 2500-7 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
TIBC | Total Iron Binding Capacity | 2500-7 |