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Test Code TEMZS Temazepam and Oxazepam, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Secondary ID

623017

Useful For

Assessing compliance

 

Monitoring for appropriate therapeutic level

 

Assessing temazepam toxicity

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Specimen Type

Serum Red

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Therapeutic concentrations

Temazepam: 600-1100 ng/mL at 1 hour

 

Cutoff concentrations by liquid chromatography tandem mass spectrometry:

Temazepam: 10 ng/mL

Oxazepam: 10 ng/mL

Interpretation

Suggested therapeutic serum temazepam concentrations range between 600 and 1100 ng/mL at 1-hour post-dose.

Method Description

The internal standard mixture containing chlordiazepoxide-d5, diazepam-d5, nordiazepam-d5, oxazepam-d5, and temazepam-d5 is added to serum samples, mixed, and centrifuged. The supernatant is diluted and injected on a liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)

Day(s) Performed

Monday, Wednesday

Report Available

3 to 7 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TEMZS Temazepam and Oxazepam, S 11024-7

 

Result ID Test Result Name Result LOINC Value
623017 Temazepam 10343-2
623018 Oxazepam 3886-9