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HelpTest Code SBL Blastomyces Antibody Immunodiffusion, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Useful For
Detection of antibodies in serum specimens from patients with blastomycosis
Method Name
Immunodiffusion
Specimen Type
SerumSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
Negative
Interpretation
A positive result is suggestive of infection, but the results cannot distinguish between active disease and prior exposure.
Routine culture of clinical specimens (eg, respiratory specimen) is recommended in cases of suspected, active blastomycosis.
Method Description
The immunodiffusion (ID) test is a qualitative test employed for the detection of precipitating antibodies present in the serum. Soluble antigens of the fungus are placed in wells of an agarose gel-filled Petri dish and the patient's serum and a control (positive) serum are placed in adjoining wells. If present, specific precipitate antibody will form precipitin lines between the wells. Their comparison to the control serum establishes the results. When performing the ID test, only precipitin bands of identity with the reference bands are significant.(Kaufman L, Kovacs JA, Reiss E: Clinical immunomycology. In: Rose NL, Conway-de Macario E, Folds JD, Lane HC, Nakamura RM, eds. Manual of Clinical Laboratory Immunology. ASM Press; 1997:587-588; Kaufman L, McLaughlin DW, Clark MJ, Blumer S: Specific immunodiffusion test for blastomycosis. Appl Microbiol. 1973;26:244-247; Williams JE, Murphy R, Standard PG, Phair JP: Serologic response in blastomycosis: diagnostic value of double immunodiffusion assay. Am Rev Resp Dis. 1981;123:209-212; Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020)
Day(s) Performed
Monday, Tuesday through Friday
Report Available
2 to 5 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86612
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SBL | Blastomyces Ab, Immunodiffusion, S | 5058-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 15125 | Blastomyces Immunodiffusion | 5058-3 |