Method Description

The Inova BIO-FLASH system uses paramagnetic beads as the solid surface upon which to bind the targeted antibody. The beads used in the rheumatoid factor (RF) IgA and IgM kits are coated with rabbit polyclonal antibodies as the capture antigen. The serum sample is diluted by the instrument and combined in a cuvette with the beads and assay buffer for 10 minutes at 37° C. The beads are magnetized, and unbound materials are washed away. Detection antibody conjugated with isoluminol is added to the cuvette for another incubation at 37° C. The RF IgA kits contain anti-human IgA conjugate, while the RF IgM kits contain anti human IgM conjugate. After an additional wash, trigger reagents are added to the cuvette to initiate a light reaction proportional to the amount of bound isoluminol conjugate. The light produced by the reaction is measured by a detector as relative light unit (RLU). The RLU is applied to the working curve on the instrument. The RF IgA is reported as chemiluminescent units (CU), while the RF IgM is reported in IU/mL traceable to WHO Reference Reagent W1066.(Package inserts: QUANTA Flash RF IgA. Inova Diagnostics, Inc; V 4, 07/2019; QUANTA Flash RF IgM. Inova Diagnostics, Inc; V 4, 07/2019)