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HelpTest Code METSP MET (SP44), Semi-Quantitative Immunohistochemistry, Manual
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.
Necessary Information
A pathology/diagnostic report and a brief history are required.
Specimen Required
Specimen Type: Tissue
Supplies: Pathology Packaging Kit (T554)
Submit:
Formalin-fixed, paraffin-embedded tissue block
OR
3 Unstained glass, "positively charged" slides with 4-microns formalin-fixed, paraffin-embedded tissue
Collection Instructions: Store slides at 2 to 8° C and send within 45 days.
Additional Information: One slide will be stained with hematoxylin and eosin and returned.
Secondary ID
623179Useful For
Aiding in the identification of normal and neoplastic MET expressing cells
Method Name
Immunohistochemistry (IHC)
Specimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Special | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Decalcified paraffin embedded tissue | Reject |
| Wet/frozen tissue | Reject |
| Cytology smears | Reject |
| Noncharged slides | Reject |
| ProbeOn slides | Reject |
| Snowcoat slides | Reject |
| Nonformalin fixed tissue, including CytoLyt, SurePath, alcohol-formalin-acetic acid (AFA), 95% ethanol, PREFER and zinc formalin fixatives | Reject |
| Nonparaffin embedded tissue | Reject |
Reference Values
An interpretive report will be provided.
Interpretation
Results are reported as percent of tumor cells with 3+ MET protein expression.
The US Food and Drug Administration approval for treatment of non-small cell lung cancer with Telisotuzumab vedotin-tllv is for tumors with strong (3+) MET expression in at least 50% of the tumor cells.
Method Description
Immunohistochemical staining and detection of MET (SP44) is performed in formalin-fixed, paraffin-embedded tissue sections using the VENTANA MET (SP44) RxDx Assay. The 4-micron tissue sections are deparaffinized, subjected to heat-induced antigen retrieval and peroxidase inhibitor, incubated with a rabbit monoclonal antibody, and visualized using a proprietary kit detection system. Sections are counterstained with hematoxylin and post-counterstained with bluing.(Package insert: VENTANA MET (SP44) RxDx Assay. Ventana Medical Systems, Inc.; 1018335US Rev A, 05/14/2025)
Results are examined microscopically by the consulting anatomic pathologist and interpreted using the manufacturer-provided interpretation guide.(Instruction manual: VENTANA MET (SP44) RxDx Assay Interpretation Guide for Non-Squamous Non-Small Cell Lung Cancer. Ventana Medical Systems, Inc.; 1018336US Rev A, 05/14/2025)
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysSpecimen Retention Time
Until reportedPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88360
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| METSP | MET (SP44), SemiQuant IHC, Manual | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 623180 | Interpretation | 59465-5 |
| 623181 | Participated in the Interpretation | No LOINC Needed |
| 623182 | Report electronically signed by | 19139-5 |
| 623183 | Material Received | 81178-6 |
| 623184 | Disclaimer | 62364-5 |
| 623185 | Case Number | 80398-1 |