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HelpTest Code Lab2205 Coenzyme Q10, Total, Plasma
Additional Codes
TQ10
Useful For
Diagnosis of primary coenzyme Q10 (CoQ10) deficiencies in some patients who are not supplemented with CoQ10
Monitoring patients receiving statin therapy
Monitoring CoQ10 status during treatment of various degenerative conditions, including Parkinson and Alzheimer diseases
Providing accurate quantitation of total CoQ10 when specimens are hemolyzed
This test is not useful for distinguishing primary CoQ10 deficiencies from acquired CoQ10 deficiencies.
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Specimen Type
Plasma HeparinOrdering Guidance
This test does not quantitate reduced coenzyme Q10 (CoQ10). For reduced CoQ10, order Q10 / Coenzyme Q10, Reduced and Total, Plasma.
The most reliable test for the diagnosis of primary defects in ubiquinone (ie, CoQ10) biosynthesis is direct measurement of CoQ10 in muscle.
Necessary Information
Patient's age is required.
Specimen Required
Patient Preparation:
Fasting: 8 hours, required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Green top (lithium or sodium heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL plasma
Collection Instructions:
1. Immediately after collection, place specimen on wet ice. Maintain on wet ice until processing.
2. Within 3 hours of collection, centrifuge, aliquot plasma into a plastic vial, and freeze immediately.
Specimen Minimum Volume
Plasma: 0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma Heparin | Frozen (preferred) | 60 days |
| Refrigerated | 7 days | |
| Ambient | 4 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
<18 years: 320-1558 mcg/L
≥18 years: 433-1532 mcg/L
Miles MV, Horn PS, Tang PH, et al. Age-related changes in plasma coenzyme Q10 concentrations and redox state in apparently healthy children and adults. Clin Chim Acta. 2004;347(1-2):139-144
Interpretation
Abnormal results are reported with a detailed interpretation including an overview of the results and their significance, a correlation to available clinical information provided with the specimen, differential diagnosis, and recommendations for additional testing when indicated and available.
Method Description
Coenzyme Q10, together with other lipid soluble substances, is extracted from plasma with cold n-propanol containing coenzyme Q10-d9 as an internal standard. An aliquot of the lipid extract is analyzed by liquid chromatography tandem mass spectrometry. The isolated coenzymeQ10 peaks corresponding to the reduced form (CoQ10H2) and the oxidized form CoQ10 are quantified by measurement off extracted and analyzed calibration curves for the respective form of Q10. Chromatography is performed using a C18 (30x2.1mm) column and total analysis time is 3.5 minutes.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Report Available
2 to 4 daysSpecimen Retention Time
1 monthPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TQ10 | Coenzyme Q10, Total, P | 27923-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 36764 | CoQ10 Total | 27923-2 |
| 36765 | Interpretation (TQ10) | 59462-2 |
| 36777 | Reviewed By | 18771-6 |
Secondary ID
63148Forms
If not ordering electronically, complete, print, and send a Biochemical Genetics Test Request (T798) with the specimen.