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Test Code LCTF Lactoferrin, Feces


Shipping Instructions


Preferred shipping temperature is frozen. Refrigerated or thawed specimens received more than 72 hours after collection will be rejected.



Specimen Required


Supplies: Stool container, Small (Random), 4 oz (T288)

Container/Tube: Stool container

Specimen Volume: 5 g

Collection Instructions:

1. Collect a fresh random fecal specimen, no preservatives.

2. If specimen is sent refrigerate, send immediately after collection.

3. If specimen cannot be sent immediately, freeze specimen and send frozen.

Additional Information:

1. Separate specimens must be submitted when multiple tests are ordered, with the exception of ELASF / Pancreatic Elastase, Feces and CALPR / Calprotectin, Feces. If only a single specimen is collected, it must be split prior to transport.

2. Testing cannot be added on to a previously collected specimen.


Secondary ID

623285

Useful For

Evaluating patients suspected of having a gastrointestinal inflammatory process

 

Distinguishing inflammatory bowel disease from irritable bowel syndrome, when used in conjunction with other diagnostic modalities, including endoscopy, histology, and imaging

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Specimen Type

Fecal

Specimen Minimum Volume

1 g

Specimen Stability Information

Specimen Type Temperature Time
Fecal Frozen (preferred) 7 days
  Ambient  72 hours
  Refrigerated  72 hours

Reject Due To

Specimens collected from diapers Reject

Reference Values

≤4.4 mcg/g (Normal)

>4.4 mcg/g (Abnormal)

Reference values apply to all ages.

Interpretation

Lactoferrin concentrations at or below 4.4 mcg/g are not suggestive of an active inflammatory process within the gastrointestinal system. For patients experiencing gastrointestinal symptoms, consider further evaluation for functional gastrointestinal disorders.

 

Fecal lactoferrin concentrations above 4.4 mcg/g are suggestive of an active inflammatory process within the gastrointestinal system. Additional diagnostic testing to determine the etiology of the inflammation is suggested.

Method Description

The TechLab LACTOFERRIN SCAN assay is a quantitative enzyme-linked immunosorbent assay for measuring concentrations of human lactoferrin in feces. Briefly, polyclonal capture antibodies specific to human lactoferrin are immobilized on a 96-well plate. Calibrators, controls, and diluted patient samples are added to the wells of the plate. If present, lactoferrin will bind to the capture antibodies on the plate. After 37° C incubation and washing, enzyme-labeled antibody conjugate is added. After another incubation and plate washing, a substrate solution is added and the plate is incubated. At the end of the assay, acidic stop solution is added to halt color development, turning the solution from blue to yellow. The absorbance of the color produced by the substrate is proportional to the amount of lactoferrin in the patient sample. Lastly, the control and patient results are calculated based on a curve generated from the kit calibrators and the dilution of the sample.(Package insert: Lactoferrin SCAN. TechLab; 07/2021)

Day(s) Performed

Tuesday

Report Available

3 to 8 days

Specimen Retention Time

Extracted feces: 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83631

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LCTF Lactoferrin, F In Process

 

Result ID Test Result Name Result LOINC Value
LCTF Lactoferrin, F In Process