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Test Code LAB9909 Q Fever Antibody Screen with Titer Reflex, Serum

Additional Codes

QFVER


Ordering Guidance


 



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Secondary ID

616832

Useful For

Screening for exposure to Coxiella burnetii, the causative agent of Q fever

 

This test should not be used as a screening procedure for the general population.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
QFP Q Fever IgM/IgG, Titer, S No No

Testing Algorithm

If the Q fever serology result is reactive, then Q fever antibody confirmation by indirect immunofluorescence will be performed at an additional charge.

 

For more information see Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages

Interpretation

Negative:

No antibodies to Q fever (Coxiella burnetii) detected. Repeat testing on a new sample collected in 2 to 3 weeks if acute Q fever is suspected.

 

Reactive:

Not diagnostic. Sample reflexed to the indirect immunofluorescence assay to determine Q fever (Coxiella burnetii) phase I and phase II IgM and IgG titers.

Method Description

The test uses microplate strips, each with 8 break-off reagent wells coated with purified native antigens from Coxiella burnetii cells in the acute and chronic phase. In the first reaction step, diluted samples are incubated in the wells. In the case of positive samples, specific IgA, IgG, or IgM antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgA-G-M (enzyme conjugate) catalyzing a color reaction.(Unpublished Mayo method)

Day(s) Performed

Sunday through Friday

Report Available

1 to 4 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86638

86638 x 4 (if applicable)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
QFEVR Q Fever Ab Scrn w/ Titer Reflex, S 23019-3

 

Result ID Test Result Name Result LOINC Value
QFEVR Q Fever Ab Scrn w/ Titer Reflex, S 23019-3

Forms

If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.