Test Code LAB9909 Q Fever Antibody Screen with Titer Reflex, Serum
Additional Codes
QFVER
Ordering Guidance
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
616832Useful For
Screening for exposure to Coxiella burnetii, the causative agent of Q fever
This test should not be used as a screening procedure for the general population.
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
QFP | Q Fever IgM/IgG, Titer, S | No | No |
Testing Algorithm
If the Q fever serology result is reactive, then Q fever antibody confirmation by indirect immunofluorescence will be performed at an additional charge.
For more information see Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology.
Special Instructions
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 7 days | |
Frozen | 7 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | Reject |
Heat-inactivated specimen | Reject |
Reference Values
Negative
Reference values apply to all ages
Interpretation
Negative:
No antibodies to Q fever (Coxiella burnetii) detected. Repeat testing on a new sample collected in 2 to 3 weeks if acute Q fever is suspected.
Reactive:
Not diagnostic. Sample reflexed to the indirect immunofluorescence assay to determine Q fever (Coxiella burnetii) phase I and phase II IgM and IgG titers.
Method Description
The test uses microplate strips, each with 8 break-off reagent wells coated with purified native antigens from Coxiella burnetii cells in the acute and chronic phase. In the first reaction step, diluted samples are incubated in the wells. In the case of positive samples, specific IgA, IgG, or IgM antibodies will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled anti-human IgA-G-M (enzyme conjugate) catalyzing a color reaction.(Unpublished Mayo method)
Day(s) Performed
Sunday through Friday
Report Available
1 to 4 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
86638
86638 x 4 (if applicable)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
QFEVR | Q Fever Ab Scrn w/ Titer Reflex, S | 23019-3 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
QFEVR | Q Fever Ab Scrn w/ Titer Reflex, S | 23019-3 |
Forms
If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.