5-Hydroxyindoleacetic Acid, Random, Urine - Medical University of South Carolina

Additional Codes

HIAAR

Ordering Guidance

This test uses a random urine collection to assess 5-hydroxyindoleacetic acid concentrations. If a 24-hour urine collection is preferred, order HIAA / 5-Hydroxyindoleacetic Acid, 24 Hour, Urine.

Necessary Information

Patient's age and sex are required.

Specimen Required

Patient Preparation:

1. Some medications could interfere with test results. The ordering provider should decide if any medications should be stopped and when they should be restarted. If clinically feasible, discontinue the following medications at least 48 hours prior to specimen collection:

-Acetaminophen (Tylenol or generic versions)

-Tryptophan containing supplements

2. For 48 hours prior to the urine collection, the patient should:

Limit the following to one serving per day:

-Fruits

-Vegetables

-Nuts

-Caffeinated beverages or foods

Supplies: Urine Tubes, 10 mL (T068)

Container/Tube: Plastic, 10-mL urine tube

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a random urine specimen.

2. Store and send refrigerate

Secondary ID

616090

Useful For

Biochemical diagnosis and monitoring of intestinal carcinoid syndrome using random urine specimens

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Specimen Type

Urine

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type	Temperature	Time	Special Container
Urine	Refrigerated (preferred)	56 days
	Frozen	365 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

5-HIAA, mg/g creatinine 99th percentile cutoff
Age	Female	Male
≤23 months	≤17.53	≤16.42
24-35 months	≤17.07	≤15.96
3 years	≤16.70	≤15.60
4 years	≤16.03	≤14.93
5 years	≤15.26	≤14.17
6 years	≤14.40	≤13.34
7 years	≤13.47	≤12.43
8 years	≤12.52	≤11.52
9 years	≤11.58	≤10.63
10 years	≤10.67	≤9.79
11 years	≤9.81	≤9.00
12 years	≤9.02	≤8.29
13 years	≤8.32	≤7.65
14 years	≤7.70	≤7.08
15 years	≤7.16	≤6.59
16 years	≤6.72	≤6.15
17 years	≤6.36	≤5.78
18 years	≤6.08	≤5.45
19 years	≤5.88	≤5.17
20 years	≤5.73	≤4.93
21 years	≤5.64	≤4.73
22 years	≤5.59	≤4.55
23 years	≤5.57	≤4.40
24 years	≤5.57	≤4.28
25 years	≤5.58	≤4.19
26 years	≤5.61	≤4.11
27 years	≤5.64	≤4.06
28 years	≤5.67	≤4.03
29 years	≤5.70	≤4.02
30 years	≤5.72	≤4.01
31 years	≤5.75	≤4.02
32 years	≤5.77	≤4.03
33 years	≤5.78	≤4.05
34 years	≤5.79	≤4.06
35 years	≤5.80	≤4.08
36 years	≤5.80	≤4.09
37 years	≤5.80	≤4.11
38 years	≤5.80	≤4.12
39 years	≤5.81	≤4.14
40 years	≤5.82	≤4.17
41 years	≤5.85	≤4.22
42 years	≤5.89	≤4.27
43 years	≤5.95	≤4.35
44 years	≤6.04	≤4.43
45 years	≤6.14	≤4.53
46 years	≤6.26	≤4.63
47 years	≤6.40	≤4.75
48 years	≤6.55	≤4.86
49 years	≤6.71	≤4.99
50 years	≤6.86	≤5.11
51 years	≤7.01	≤5.24
52 years	≤7.15	≤5.37
53 years	≤7.29	≤5.51
54 years	≤7.41	≤5.64
55 years	≤7.52	≤5.78
56 years	≤7.62	≤5.91
57 years	≤7.71	≤6.05
58 years	≤7.80	≤6.17
59 years	≤7.88	≤6.29
60 years	≤7.95	≤6.41
61 years	≤8.02	≤6.51
62 years	≤8.09	≤6.60
63 years	≤8.15	≤6.69
64 years	≤8.21	≤6.76
65 years	≤8.28	≤6.82
66 years	≤8.34	≤6.88
67 years	≤8.40	≤6.93
68 years	≤8.46	≤6.97
69 years	≤8.52	≤7.00
70 years	≤8.58	≤7.03
71 years	≤8.65	≤7.06
72 years	≤8.71	≤7.08
73 years	≤8.77	≤7.10
74 years	≤8.82	≤7.11
75 years	≤8.86	≤7.11
76 years	≤8.90	≤7.11
77 years	≤8.92	≤7.10
78 years	≤8.93	≤7.09
79 years	≤8.93	≤7.07
80 years	≤8.92	≤7.05
81 years	≤8.90	≤7.02
82 years	≤8.88	≤7.00
83 years	≤8.86	≤6.98
84 years	≤8.85	≤6.97
85 years	≤8.84	≤6.95
86 years	≤8.84	≤6.94
87 years	≤8.84	≤6.94
88 years	≤8.84	≤6.94
>or= 89 years	≤8.85	≤6.93

Interpretation

If pharmacological and dietary artifacts have been ruled out, an elevated excretion of 5-hydroxyindoleacetic acid is a probable indicator of the presence of a serotonin-producing tumor.

Method Description

5-Hydroxyindoleacetic acid (5-HIAA) is measured by solid phase extraction of an aliquot from a random urine collection and liquid chromatography tandem mass spectrometry analysis. 5-HIAA is quantitated using a custom synthesized stable isotope labeled internal standard (d6-5-HIAA) from calibration over a concentration range 0.5 to 150 mg/L.(Kroll CA, Magera MJ, Helgeson JK, et al. A liquid chromatography-tandem mass spectrometry method for the determination of 5-hydroxyindole-3-acetic acid in urine. Clin Chem. 2002;48:2049-2051; Calanchini M, Tadman M, Krogh J, Fabbri A, Grossman A, Shine B. Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection. Endocr Connect. 2019;8(8):1082-1088)

Day(s) Performed

Monday through Friday

Report Available

2 to 4 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83497

LOINC Code Information

Test ID	Test Order Name	Order LOINC Value
HIAAR	5-HIAA, Random, U	11145-0

Result ID	Test Result Name	Result LOINC Value
616090	5-HIAA, Random, U	11145-0

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Test Code LAB9856 5-Hydroxyindoleacetic Acid, Random, Urine