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HelpTest Code LAB9838 Phosphatidylethanol Confirmation, Blood
Additional Codes
PETH
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 mL
Collection Instructions:
1. Do not use alcohol to clean arm. Use alternatives such as Betadine or ChloraPrep to cleanse arm before collecting any specimen.
2. Do not centrifuge.
3. Send whole blood specimen in original tube. Do not aliquot.
Secondary ID
617480Useful For
Verifying abstinence or use of ethanol, especially in liver transplant candidates/patients
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Specimen Type
Whole Blood EDTASpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Frozen (preferred) | 28 days | |
| Refrigerated | 14 days |
Reject Due To
| Gross lipemia | OK |
| Gross icterus | OK |
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Send in original container, Refrigerated
Off-campus collections: DO NOT SPIN! DO NOT SHARE! Specimen to be stored/transported Refrigerated.
Reference Values
Negative (<10 ng/mL)
PEth 16:0/18:1 (POPEth): Lower limit of quantification =10 ng/mL
PEth 16:0/18:2 (PLPEth): Lower limit of quantification =10 ng/mL
Interpretation
POPEth (1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol):
A result of 20 ng/mL to 200 ng/mL is considered evidence of moderate ethanol consumption, while results over 200 ng/mL indicate heavy ethanol consumption. However, the Center for Substance Abuse Treatment advises caution in interpretation and use of biomarkers alone to assess alcohol use. Results should be interpreted in the context of all available clinical and behavioral information.(2)
PLPEth (1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphoethanol):
There are no current clinical reference limits for this phosphatidylethanol (PEth) homologue.
PEth 16:0/18:1 (POPEth)
Less than 10 ng/mL: Not detected
10-19 ng/mL: Abstinence or light alcohol consumption (<2 drinks per day for several days a week)
20-200 ng/mL: Moderate alcohol consumption (up to 4 drinks per day for several days a week)
Greater than 200 ng/mL: Heavy alcohol consumption or chronic alcohol use (at least 4 drinks per day several days a week)
PEth 16:0/18:2 (PLPEth): Reference ranges are not well established.
Method Description
The received whole blood sample is diluted and mixed with internal standard and clinical laboratory reagent water, extracted using supported liquid extraction, and analyzed by an in-house developed liquid chromatography tandem mass spectrometry method.(Unpublished Mayo method)
Day(s) Performed
Monday through Sunday
Report Available
2 to 5 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
80321
G0480 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PETH | Phosphatidylethanol Confirmation, B | 101506-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 617481 | PEth 16:0/18:1 (POPEth) by LC-MS/MS | 97607-6 |
| 617482 | PEth 16:0/18:2 (PLPEth) by LC-MS/MS | 97606-8 |
| 617483 | PEth Interpretation | 69050-3 |
Forms
If not ordering electronically, complete, print, and send 1 of the following with the specimen:
-Therapeutics Test Request (T831)
-General Test Request (T239)
-Renal Diagnostics Test Request (T830)