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Test Code LAB9800 SC5b-9 Level Terminal Complement Complex, Plasma

Additional Codes

C5B9


Specimen Required


Patient Preparation:

1. Fasting preferred.

2. Do not collect a specimen for the 48 hours following a plasma exchange.

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Centrifuge between 1000-2000 x g for 10 minutes at 4° C and aliquot plasma into plastic vial.

3. Freeze specimen within 30 minutes.


Secondary ID

616921

Useful For

Detecting increased complement activation

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis OK
Gross lipemia Reject
Gross icterus OK

Reference Values

≤250 ng/mL

Interpretation

Elevated concentrations of soluble C5b-9 suggest recent or ongoing activation of the complement system, while normal and low concentrations suggest that the complement system has not been excessively activated.   A panel of complement tests may be clinically indicated to further identify the extent of the complement activation, along with the information of which pathway is most dysregulated.

Method Description

Microtiter plates are coated with monoclonal antibody specific to the C9 ring of the soluble C5b-9 (sC5b-9) complex. Controls, standards, and patient samples are exposed to the plate. After washing the plate, a horseradish peroxidase-conjugated anti-sC5b-9 complex antibody is added followed by a substrate to initiate color change.(Package insert: MicroVue SC5b-9 Plus EIA Kit. Quidel Corporation; 0980EN02, 09/2021)

Day(s) Performed

Tuesday, Friday

Report Available

3 to 5 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86160

LOINC Code Information

Test ID Test Order Name Order LOINC Value
C5B9 SC5b-9 Complement, P 93244-2

 

Result ID Test Result Name Result LOINC Value
616921 SC5b-9 Complement, P 93244-2