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HelpTest Code LAB9800 SC5b-9 Level Terminal Complement Complex, Plasma
Additional Codes
C5B9
Ordering Guidance
If testing to evaluate C5b-9 in the context of atypical hemolytic uremic syndrome is desired, see AHUSD / Atypical Hemolytic Uremic Syndrome Complement Panel, Serum and Plasma.
Specimen Required
Patient Preparation:
1. Fasting preferred but not required.
2. Do not collect specimens for at least 48 hours following plasma exchange.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Lavender top (K2 EDTA)
Acceptable: Lavender top (K3 EDTA), light-blue top (sodium citrate)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions:
1. Immediately after specimen collection, place the tube on wet ice.
2. Centrifuge between 1000 and 2000 x g for 10 minutes at 4° C and aliquot plasma into a plastic vial.
3. Within 30 minutes of centrifugation, freeze specimen. Sample must be placed on dry ice if not frozen immediately.
NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the sample is kept on ice before centrifugation, and immediately afterward, the plasma is aliquoted and frozen.
Secondary ID
616921Useful For
Detecting increased complement activation
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Specimen Type
Plasma EDTASpecimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 14 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
≤250 ng/mL
Interpretation
Elevated concentrations of soluble C5b-9 suggest recent or ongoing activation of the complement system, while normal and low concentrations suggest that the complement system has not been excessively activated.
A panel of complement tests may be clinically indicated to further identify the extent of the complement activation, along with the information of which pathway is most dysregulated.
Method Description
Microtiter plates are coated with monoclonal antibody specific to the C9 ring of the soluble C5b-9 (sC5b-9) complex. Controls, standards, and patient samples are exposed to the plate. After washing the plate, a horseradish peroxidase-conjugated anti-sC5b-9 complex antibody is added followed by a substrate to initiate color change.(Package insert: MicroVue SC5b-9 Plus EIA Kit. Quidel Corporation; PIA020004EN00, 06/2022)
Day(s) Performed
Varies
Report Available
3 to 5 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86160
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| C5B9 | SC5b-9 Complement, P | 93244-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 616921 | SC5b-9 Complement, P | 93244-2 |