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HelpTest Code LAB9750 Echinococcus Antibody, IgG, Serum
Additional Codes
ECHNG
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Secondary ID
616731Useful For
Detection of antibodies to Echinococcus granulosus
Method Name
Enzyme Immunoassay (EIA)
Specimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated | Reject |
Reference Values
Negative
Reference values apply to all ages.
Interpretation
Negative:
The absence of antibodies to Echinococcus species suggests that the individual has not been exposed to this cestode. A single negative result should not be used to rule out infection (see Cautions).
Positive:
Results suggest infection with Echinococcus. False-positive results may occur in settings of infection with other helminths or in patients with chronic immune disorders. Results should be considered alongside other clinical findings (eg, characteristic findings on imaging) and exposure history.
Method Description
The Bordier Echinococcus granulosus IgG ELISA is an enzymatically amplified sandwich-type immunoassay. After a blocking step, diluted serum and controls are incubated in antigen coated microtiter wells, then washed and incubated with anti-human IgG antibody labeled with protein A-alkaline phosphatase conjugate. After a washing step, the wells are incubated with the colorless pNPP substrate. The enzyme converts the substrate to a yellow product. The reaction is stopped by adding potassium phosphate and the degree of enzymatic turnover is determined by absorbance measured at 405 nm on the ELISA microplate reader on the Dynex Agility. OD results of the patient sample are compared to the assay calibrator to establish a final index value for qualitative interpretation.(Package insert: Bordier Echinococcus granulosus IgG ELISA, Bordier Affinity Products SA; 01/2018)
Day(s) Performed
Tuesday
Report Available
Same day/1 to 7 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86682
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ECHNG | Echinococcus Ab, IgG, S | 32171-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ECHNG | Echinococcus Ab, IgG, S | 32171-1 |
Forms
If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.