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Test Code LAB9672 Phospholipase A2 Receptor, Immunofluorescence, Serum

Important Note

REFLEX TEST FOR PMND1

Additional Codes

PLA2I

PLA2R


Ordering Guidance


This test can be used in a complementary fashion with a quantitative enzyme-linked immunosorbent assay to confirm a positive result, especially if it is a low-level titer. Since the immunofluorescence is more sensitive, it can also be used to detect or follow patients with low level antibodies not detected by ELISA.  



Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL 

Collection Instructions: Centrifuge and aliquot serum into a plastic vial within 2 hours of collection.


Secondary ID

607367

Useful For

Distinguishing primary from secondary membranous nephropathy in patients with low levels of anti-phospholipase A2 receptor (PLA2R) antibodies

 

Screening for anti-PLA2R antibodies

 

Monitoring patients with membranous nephropathy at very low antibody titers

Method Name

Indirect Immunofluorescence Assay (IFA)

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  8 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Reference Values

Negative

Interpretation

According to the manufacturer's package insert, the EUROIMMUN Anti-PLA2R indirect immunofluorescence assay was positive in 77.1% of patients with biopsy proven primary membranous nephropathy (pMN). This corresponds well with published literature that approximately 70% of patients with pMN will have anti-phospholipase A2 receptor antibodies.

Method Description

Diluted patient samples are incubated with combinations of substrates. If the reaction is positive, specific antibodies of classes IgA, IgG, and IgM attach to the antigens. In a second step, the attached antibodies are stained with fluorescein-labelled antihuman antibodies and made visible with a fluorescence microscope.(Package insert: Anti-Phospholipase A2 Receptor (PLA2R) IFA EUROPattern Kit, EUROIMMUN US;V 11/23/2021)

Day(s) Performed

Monday, Wednesday, Friday

Report Available

3 to 7 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86255

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLA2I PLA2R, Immunofluorescence, S 82991-1

 

Result ID Test Result Name Result LOINC Value
PLA2I PLA2R, Immunofluorescence, S 82991-1

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.