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Test Code LAB8994 SARS-COV-2, Influenza A and B, RSV PCR

Additional Codes:

SARS-CoV-2, Flu A, Flu B, and RSV PCR

Collection:

Specimen:  Nasopharyngeal swabs, nasal aspirates/washes, Nasal swabs, and mid-turbinate swabs.

Container:  Nasopharyngeal (NP) swabs - Universal Transport Media.  Submit nasal aspirates/washes  in sterile container.

Volume:  4 mL; Minimum 1 mL.

Storage Instructions:

On-campus collections:  Send specimen to Lab immediately after collection.  Refrigerate or keep on wet ice if specimen cannot be sent immediately.

Off-campus collections:  Ship by overnight delivery with frozen cold packs.

Turnaround Time (TAT):

Reported within 24-48 hours of receipt in laboratory

Methodology

Cepheid GeneXpert

Request Form:

Routine Laboratory Request Form

Send To:

Central Processing – EH318 University Extension Hospital (former Children’s Hopsital)

Reference Interval:

SARS-CoV-2 Negative; Flu A Negative; Flu B Negative; RSV Negative.

 

Interpretive Data

Assay methodology is real time polymerase chain reaction (PCR).

The PCR primer targets for Coronavirus 2 (SARS COVID-19) are virus nucleocapsid (N) and envelope (E) and RNA-dependent RNA polymerase (RdRP) genes. 

The PCR primer targets for Influenza A Virus (Flu A) assay are virus matrix, basic polymerase 2, and acidic protein genes.

The PCR primer targets for Influenza B virus (Flu B) are the virus matrix and nonstructural protein genes.

The PCR primer targets for Respiratory Syncytial Virus (RSV) are the RSV A and RSV B nucleocapsid genes.

 

Negative:  Target(s) are not detected. 
Positive:  Target(s) are detected 
No Result-Repeat Test:  Testing was performed twice with no results; consider collecting another specimen from the patient if warranted.