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HelpTest Code LAB8982 Brucella Total Antibody Confirmation, Agglutination, Serum
Additional Codes
BRUTA
Useful For
Diagnosis of brucellosis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 14 days |
Reference Values
<1:80
Day(s) Performed
Wednesday, Friday
CPT Code Information
86622
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BRUTA | Brucella Ab, Agglutination, S | 19053-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8112 | Brucella Ab, Agglutination, S | In Process |
Interpretation
The Centers for Disease Control and Prevention recommends that specimens testing positive or equivocal for IgG or IgM by a screening enzyme immunoassay (EIA) be confirmed by a Brucella-specific agglutination method.
Titers below 1:80 are seen in normal, healthy populations. Titers of 1:80 or greater are often considered clinically significant (1); however, a 4-fold or greater increase in titers between acute and convalescent phase sera is required to diagnose acute infection.
Positive results by a screening EIA that are not confirmed by Brucella-specific agglutination may represent false-positive screening results. If clinically indicated, a new specimen should be tested after 7 to 14 days.
Method Description
Serially diluted serum is added to an antigen prepared from Brucella abortus strain 1119-3. Agglutination or flocculation is assessed after incubation at 37° C for 48 hours.(Package insert: Animal and Plant Health Inspection Service National Veterinary Services Laboratories, Kirsh D. US Dept of Health, Education, and Welfare; 1973; Cooke FJ, Slack MPE. Gram-negative coccobacilli. In: Cohen J, Powderly WG, Opal SM, eds. Infectious Diseases. 4th ed. Elsevier; 2017:1611-1627)
Report Available
2 to 7 daysSpecimen Retention Time
14 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Method Name
Agglutination
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.