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HelpTest Code LAB8898 Trypanosoma cruzi Total Antibody, Enzyme-Linked Immunosorbent Assay, Serum
Additional Codes
TCRUZI
CHAGAS
Specimen Required
Only orderable as part of a profile. For more information see CRUZI / Trypanosoma cruzi (Chagas) Antibody Panel, Serum.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
615244Useful For
Diagnosis of chronic Trypanosoma cruzi infection (Chagas disease)
Method Name
Only orderable as part of a profile. For more information see CRUZI / Trypanosoma cruzi (Chagas) Antibody Panel, Serum.
Enzyme-Linked Immunosorbent Assay (ELISA)
Specimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
Only orderable as part of a profile. For more information see CRUZI / Trypanosoma cruzi (Chagas) Antibody Panel, Serum.
Negative
Reference values apply to all ages.
Interpretation
|
CHAGS result |
CHAGL result |
Interpretive comment |
|
Positive |
Positive |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by two separate methods, suggesting current or past infection. Results should be interpreted alongside clinical presentation and exposure history. |
|
Positive |
Negative |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by one of two assays. Discordant results can be resolved through submission of a new sample for testing or through additional testing at a public health laboratory. |
|
Positive |
Invalid |
Submission of a new sample is recommended to resolve discordant results. |
|
Indeterminate |
Positive |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by one of two assays. Discordant results can be resolved through submission of a new sample for testing or through additional testing at a public health laboratory. |
|
Indeterminate |
Negative |
Submission of a new sample is recommended to resolve discordant results. |
|
Indeterminate |
Invalid |
Submission of a new sample is recommended to resolve discordant results. |
|
Negative |
Positive |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by one of two assays. Discordant results can be resolved through submission of a new sample for testing or through additional testing at a public health laboratory. |
|
Negative |
Negative |
No antibodies to Trypanosoma cruzi (Chagas disease) detected. False negative results may occur in patients tested within 4 weeks of infection. |
|
Negative |
Invalid |
Submission of a new sample is recommended to resolve discordant results. |
Method Description
The Wiener Chagatest ELISA (enzyme-linked immunosorbent assay) recombinante v.3.0 test kit is a qualitative technique for the detection of anti-Trypanosoma cruzi antibodies. The sample is diluted in the support in which the recombinant antigen (1, 2, 13, 30, 36, and SAPA) is immobilized (3rd generation method). These antigens are obtained by DNA recombinant techniques starting from specific proteins from the epimastigote and trypomastigote stages of the T cruzi corresponding to highly-conserved zones among different strains. The technology used allows us to ensure an antigenic mixture of known and permanent composition batch to batch, giving reproducible, specific, and highly-sensitive results. If the sample contains specific antibodies, these will form a complex with the antigens and will remain bound to the support. The unbound fraction is eliminated by washing, after which antihuman immunoglobulin antibodies conjugated to peroxidase are added. If a reaction is produced in the first step of the process, the conjugate is bound. After a new wash, the enzymatic substrate is added. If bound conjugate is present, a light-blue color is developed. The reaction is stopped by adding sulfuric acid, and the color changes to yellow.(Package insert: Chagatest ELISA recombinante v.3.0. Wiener Laboratorios S.A.I.C.; 801146000/00)
Day(s) Performed
Monday
Report Available
Same day/1 to 8 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86753
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CHAGS | T. cruzi Total Ab, EIA, S | 57320-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CHAGS | T. cruzi Total Ab, EIA, S | 57320-4 |