Test Code LAB8898 Trypanosoma cruzi Total Antibody, Enzyme-Linked Immunosorbent Assay, Serum

Important Note

This test is NOT part of the Stem cell Panel

Additional Codes

TCRUZI

CHAGAS

Specimen Required

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Secondary ID

615244

Useful For

Diagnosis of chronic Trypanosoma cruzi infection (Chagas disease)

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Specimen Type

Serum

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type	Temperature	Time	Special Container
Serum	Frozen	14 days

Reject Due To

Gross hemolysis	Reject
Gross lipemia	Reject

Reference Values

Negative

Reference values apply to all ages.

Interpretation

Negative:

No antibodies to Trypanosoma cruzi (Chagas disease) detected. Antibodies to T cruzi may be absent during acute infection, therefore a negative result cannot be used to rule out recent infection. Repeat testing in 3 to 4 weeks is recommended if clinically indicated.

Indeterminate:

Repeat testing in 2 to 3 weeks if clinically indicated.

Reactive:

Antibodies to T cruzi detected. Result is not diagnostic. Supplemental testing by a second, different T cruzi serologic assay is recommended.

Method Description

The Wiener Chagatest ELISA recombinante v.3.0 test kit is a qualitative technique for the detection of anti-Trypanosoma cruzi antibodies. The sample is diluted in the support in which the recombinant antigen (1, 2, 13, 30, 36, and SAPA) is immobilized (3rd generation method). These antigens are obtained by DNA recombinant techniques starting from specific proteins from the epimastigote and trypomastigote stages of the T cruzi corresponding to highly conserved zones among different strains. The technology used allows us to ensure an antigenic mixture of known and permanent composition batch to batch, giving reproducible, specific, and highly sensitive results. If the sample contains specific antibodies, these will form a complex with the antigens and will remain bound to the support. The unbound fraction is eliminated by washing, after which antihuman immunoglobulin antibodies conjugated to peroxidase are added. If a reaction is produced in the first step of the process, the conjugate is bound. After a new wash, the enzymatic substrate is added. If bound conjugate is present, a light-blue color is developed. The reaction is stopped by adding sulfuric acid, and the color changes to yellow.(Package insert: Chagatest ELISA recombinante v.3.0. Wiener Laboratorios S.A.I.C.; 801146000/00)

Day(s) Performed

Monday

Report Available

Same day/1 to 8 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86753

LOINC Code Information

Test ID	Test Order Name	Order LOINC Value
CHAGS	T. cruzi Total Ab, EIA, S	57320-4

Result ID	Test Result Name	Result LOINC Value
CHAGS	T. cruzi Total Ab, EIA, S	57320-4

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

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