Home
HelpTest Code LAB8885 Sickle Solubility, Blood
Additional Codes
SDEX
Useful For
Screening for presence or absence of hemoglobin (Hb) S (sickle cell disease)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAOrdering Guidance
This is a screening test only. For quantification of hemoglobin S, order HBEL1 / Hemoglobin Electrophoresis Evaluation, Blood.
Necessary Information
1. Patient's age is required.
2. Include recent transfusion information.
Specimen Required
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD solution B), green top (heparin)
Specimen Volume: 1 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in the original tube. Do not aliquot.
Specimen Minimum Volume
0.5 mL
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Do NOT Spin!
Off-campus collections: Do NOT Spin! Specimen to be stored/transported at Refrigerated temp.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Refrigerated | 14 days |
Reference Values
Negative
Day(s) Performed
Monday through Friday
CPT Code Information
85660
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SDEX | Sickle Solubility, B | 6864-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 9180 | Sickle Solubility, B | 6864-3 |
Interpretation
A positive result should be followed by a complete hemoglobin (Hb) evaluation (HBEL1 / Hemoglobin Electrophoresis Evaluation, Blood) to confirm the presence and concentration of Hb S.
Method Description
Hemoglobin S reduced by dithionite is insoluble in concentrated inorganic buffers, and thus, the solution is so turbid that newsprint cannot be read through a tube containing the hemolysate and reagents.(Fairbanks VF, Klee GG: Biochemical aspects of hematology. In: Burtis CA, Ashwood ER, eds. Tietz Textbook of Clinical Chemistry. 3rd ed. WB Saunders Company; 1999: 1678-1679; Greene DN, Vaughn CP, Crews BO, Agarwal AM. Advances in the detection of hemoglobinopathies. Clinica Chimica Acta. 2015; 439:50-57)
Report Available
1 to 4 daysSpecimen Retention Time
7 daysReject Due To
| Gross hemolysis | Reject |
Method Name
Hemoglobin S Solubility
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.