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HelpTest Code LAB8877 Leukocyte Adhesion Deficiency Type 1, CD11a/CD18 and CD11b/CD18 Complex Immunophenotyping, Blood
Additional Codes
LAD1
Useful For
Aiding in the diagnosis of leukocyte adhesion deficiency syndrome type 1, primarily in patients younger than 18 years
CD11a, CD11b, and CD18 phenotyping
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAShipping Instructions
Testing is performed Monday through Friday. Specimens not received by 4 p.m. (CST) on Friday may be canceled.
Draw and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of draw.
Specimens arriving on the weekend and observed holidays may be canceled.
Necessary Information
Date and time of collection and healthcare professional name and phone number are required.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 5 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Additional Information: For serial monitoring, it is recommended that specimens are collected at the same time of day.
Specimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Off-campus collections:
Send specimen in original tube. Do not aliquot. DO NOT SPIN! DO NOT SHARE!
Specimen to be stored/transported Ambient
Reference Values
Normal (reported as normal or absent expression for each marker)
Day(s) Performed
Monday through Friday
CPT Code Information
86356 x 3
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LAD1 | Leukocyte Adhesion Def. Type 1, B | 94266-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 81155 | Leukocyte Adhesion Deficiency, B | No LOINC Needed |
| 430 | CD11a | 94268-0 |
| 388 | CD11b | 94267-2 |
| 431 | CD18 | 94265-6 |
| 432 | LAD Interpretation | 69052-9 |
Interpretation
The report will include a summary interpretation of the presence or reduction in the level of expression of the individual markers (CD11a, CD11b, and CD18). Expression of the individual markers provides indirect information on the presence or absence of the CD11a/CD18 and CD11b/CD18 complexes.
Specimens obtained from patients with leukocyte adhesion deficiency syndrome type 1 (LAD-1) show significant reduction (moderate phenotype) or near absence (severe phenotype) of CD18 and its associated molecules, CD11a and CD11b, on neutrophils and other leukocytes.
CD11c expression also is low in LAD-1. The analytical sensitivity of the CD11c assay is insufficient to allow interpretation of CD11c surface expression. Therefore, this test is only for the expression of CD18, CD11a, and CD11b.
Method Description
Flow cytometric immunophenotyping of peripheral blood is performed to evaluate the presence or absence of the CD11/CD18 complex using monoclonal antibodies directed against the CD11 isoforms, CD11a and CD11b, and CD18 antigens.(O'Gorman MR, McNally AC, Anderson DC, et al. A rapid whole blood lysis technique for the diagnosis of moderate or severe leukocyte adhesion deficiency [LAD]. Ann NY Acad Sci. 1993;677:427-430)
Report Available
3 to 4 daysSpecimen Retention Time
4 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Method Name
Flow Cytometric Immunophenotyping