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Test Code LAB8653 Kingella kingae, Molecular Detection, PCR, Varies

Additional Codes

KKRP

Secondary ID

65201

Useful For

Aiding in the diagnosis of Kingella kingae infection using tissue or synovial fluid specimens

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Kingella kingae DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Synovial fluid

Preferred: Lavender top (EDTA)

Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Send specimen in original tube (preferred).

Specimen Stability Information: Refrigerated (preferred) <7 days /Frozen <7 days

 

Specimen Type: Fresh tissue or biopsy

Sources: Bone, joint, synovium, heart valve, aorta, or endocardium

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm(3)- approximately the size of a pencil eraser

Collection Instructions:

1. Collect fresh tissue specimen.

2. Submit tissue only, do not add fluid to tissue

3. Refrigerate or freeze specimen.

Specimen Stability Information: Refrigerated (preferred) <7 days/ Frozen <7 days

 

Preferred Paraffin-embedded tissue block:

Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)

Sources: Bone, joint, synovium, heart valve, aorta, or endocardium

Supplies: Tissue Block Container (T553)

Container/Tube: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block to be cut and returned.

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Acceptable Paraffin-embedded tissue block:

Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)

Sources: Bone, joint, synovium, heart valve, aorta, or endocardium

Container/Tube: Sterile container for each individual cut section (scroll).

Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.

Specimen Stability Information: Ambient (preferred)/Refrigerated


Specimen Minimum Volume

Fluid/fresh tissue or biopsy: See Specimen Required
Paraffin-embedded tissue block: Two 10-micron sections

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies
Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318 
 
Off-campus collections: 
Centrifuge and aliquot within ___ minutes/hours.  N/A
Specimen to be stored/transported at (See below):
·    Synovial fluid:  Refrigerated 
·    Fresh tissue or biopsy:  Refrigerated
·    Paraffin-embedded tissue block:  Ambient

Reject Due To

Tissue in formalin, formaldehyde, or acetone Decalcified bone Bone marrow
Slides		 Reject

Reference Values

Not applicable

Interpretation

A positive result indicates the presence of Kingella kingae DNA.

 

A negative result indicates the absence of detectable K kingae DNA but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of K kingae DNA in quantities less than the limit of detection of the assay.

Method Description

Nucleic acid is extracted from the specimen using the automated MagNA Pure instrument. Target specific primers are used to amplify the rxtB gene region of Kingella kingae; amplification is monitored by detecting fluorescence produced by target specific fluorescence resonance energy transfer hybridization probes. This real-time polymerase chain reaction (PCR) takes place on a LightCycler instrument. Detection of the K kingae target is performed through melting curve analysis using the LightCycler software.(Cockerill FR, Uhl JR: Applications and challenges of real-time PCR for the clinical microbiology laboratory. In: Reischl U, Wittwer C, Cockerill F, eds. Rapid Cycle Real-Time PCR Methods and Applications. Springer-Verlag, 2002:3-27; Zbinden R: Aggregatibacter, Capnocytophaga, Eikenella, Kingella, Pasteurella, and other fastidious or rarely encountered gram-negative rods. In: Carroll K, Pfaller M, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019:656-669)

Day(s) Performed

Monday through Friday

Report Available

2 to 7 days

Specimen Retention Time

1 week

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
KKRP Kingella kingae PCR 65809-6

 

Result ID Test Result Name Result LOINC Value
KKSRC Specimen Source 31208-2
48324 Kingella kingae PCR 65809-6

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244)with the specimen.