Home
HelpTest Code LAB8648 T-Cell Receptor Gene Rearrangement, PCR, Varies
Additional Codes
TCGRV T Cell Clonality
Useful For
Determining whether a T-cell population is polyclonal or monoclonal using body fluid or tissue specimens
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesShipping Instructions
Body fluid or spinal fluid specimens must arrive within 4 days of collection.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Container/Tube: Sterile container
Specimen Volume: At least 5 mL
Collection Instructions:
1. If the volume is large, pellet cells prior to sending.
2. Send less volume at ambient temperature or as a frozen cell pellet.
Specimen Stability Information:
Body fluid: Ambient 4 days/Refrigerated/Frozen
Cell pellet: Frozen
Specimen Type: Paraffin-embedded bone marrow aspirate clot
Container/Tube: Paraffin block
Specimen Stability Information: Ambient
Specimen Type: Frozen tissue
Container/Tube: Plastic container
Specimen Volume: 100 mg
Collection Instructions: Freeze tissue within 1 hour of collection.
Specimen Stability Information: Frozen
Specimen Type: Paraffin-embedded tissue
Container/Tube: Paraffin block
Specimen Stability Information: Ambient
Specimen Type: Tissue slides
Container/Tube: Unstained tissue slides
Specimen Volume: 10 slides
Specimen Stability: Ambient
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 5 to 10 mL
Specimen Stability Information: Ambient 4 days/Refrigerated
Specimen Type: Extracted DNA
Container/Tube: 1.5- to 2-mL tube with indication of volume and concentration of DNA
Specimen Volume: Entire specimen
Collection Instructions:
1. Label specimen as extracted DNA and source of specimen
2. Indicate volume and concentration of DNA on label
Specimen Stability Information: Refrigerated/Ambient
Specimen Minimum Volume
Body fluid or Spinal fluid: 1 mL
Tissue: 50 mg
Extracted DNA: 50 microliters at 20 ng/mcL
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Off-campus collections:
· Body fluid: Ambient/Cell pellet - Frozen
· Spinal fluid: Ambient
· Paraffin embedded bone marrow aspirate clot: Ambient
· Paraffin embedded tissue: Ambient
· Frozen tissue: Frozen
· Tissue slides: Ambient
· Extracted DNA from blood or bone marrow: Refrigerated
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Special Instructions
Reference Values
An interpretive report will be provided.
Positive, negative, or indeterminate for a clonal T-cell population
Day(s) Performed
Monday through Friday
CPT Code Information
81340-TCB (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s), using amplification methodology (eg, PCR)
81342-TCG (T cell receptor, gamma) (eg, leukemia and lymphoma), gene rearrangement analysis, evaluation to detect abnormal clonal population(s)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TCGRV | T Cell Receptor Gene Rearrange, V | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 19936 | Final Diagnosis: | 22637-3 |
| MP016 | Specimen: | 31208-2 |
| 608953 | Signing Pathologist | 19139-5 |
Interpretation
An interpretive report will be provided.
Results will be characterized as positive, negative, or indeterminate for a clonal T-cell population.
In the appropriate clinicopathologic setting, a monoclonal result is associated with a neoplastic proliferation of T cells (see Cautions).
Method Description
Genomic DNA is extracted from the tissue source. T-cell receptor beta (TCRB) and T-cell receptor gamma (TCRG) loci (official designations TRB and TRG, respectfully) are amplified by polymerase chain reaction (PCR) using a multiplex primer method based on the BIOMED-2 strategy. Specific primers are labeled with fluorochrome dyes, permitting precise fragment sizing of PCR products by capillary gel electrophoresis using a genetic analyzer. Each amplified locus is assessed for gene rearrangement patterns and an overall interpretation of the assay is made with regards to the presence or absence of a monoclonal population.(Unpublished Mayo method)
Report Available
7 to 14 daysSpecimen Retention Time
Extracted DNA: 3 monthsReject Due To
| Bone marrow core biopsies Paraffin shavings |
Reject |
Method Name
Polymerase Chain Reaction (PCR)
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.