FLT3 Mutation Analysis, Varies - Medical University of South Carolina

Additional Codes

FLT

Useful For

Prognostic indication for some patients with acute myeloid leukemia

This test should not be used to monitor residual disease following treatment.

Special Instructions

Method Name

Polymerase Chain Reaction (PCR)/Capillary Electrophoresis

Specimen Type

Varies

Ordering Guidance

This test is intended to be used as a prognostic test at diagnosis and should not be used to monitor residual disease following treatment.

Shipping Instructions

Specimen must arrive within 7 days of collection.

Necessary Information

The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date and time of collection

4. Specimen source

Specimen Required

Submit only 1 of the following specimens:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA) or yellow top (ACD)

Specimen Volume: 3 mL

Collections Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube. Do not aliquot.

3. Label specimen as blood.

Specimen Stability Information: Ambient (preferred)/Refrigerate

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) or yellow top (ACD)

Specimen Volume: 2 mL

Collections Instructions:

1. Invert several times to mix bone marrow.

2. Send specimens in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability Information: Ambient (preferred)/Refrigerate

Specimen Type: Extracted DNA from blood or bone marrow

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. Label specimen as extracted DNA from blood or bone marrow

2. Indicate volume and concentration of DNA on the label.

Specimen Stability Information: Frozen (preferred)/Refrigerate/Ambient

Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318.

Whole blood: Do NOT Spin! Send in original tube, Ambient.

Bone Marrow: Send in original tube, Ambient.

Extracted DNA: Send in 1.5-2.0 mL tube, Frozen.

Off-campus collections: Do NOT Spin!
Specimen to be stored/transported at Ambient for Whole Blood or Bone Marrow, or Frozen for Extracted DNA.

Specimen Minimum Volume

Blood, Bone Marrow: 1 mL
Extracted DNA: 50 mcL at 20 ng/mcL concentration

Specimen Stability Information

Specimen Type	Temperature	Time	Special Container
Varies	Varies	7 days

Reject Due To

Gross hemolysis	Reject
Bone marrow biopsies, slides, paraffin shavings Moderately to severely clotted	Reject

Reference Values

An interpretive report will be provided.

Interpretation

An interpretive report will be issued indicating whether the FLT3 internal tandem duplication (ITD), D835 alteration, or both were detected.

Variant status will be indicated as positive or negative. If ITD positive, an allelic ratio will be reported.

Method Description

This polymerase chain reaction (PCR)-based assay is designed to detect the presence of 2 separate variants in FLT3: internal tandem duplication (ITD) of coding sequence for the intracellular juxtamembrane domain and point alterations in the codon for Asp835 (D835). Genomic DNA is extracted from nucleated cells in the sample. A multiplex PCR is then performed using 2 sets of primers. One primer in each set is labeled with a fluorescent dye to aid in PCR-fragment analysis. The PCR products are then analyzed using capillary electrophoresis.(Unpublished Mayo method)

Day(s) Performed

Monday through Saturday

Report Available

3 to 6 days

Specimen Retention Time

Whole blood, bone marrow: 2 weeks; Extracted DNA: 3 months

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81245-FLT3 (fms-related tyrosine kinase) (eg, acute myeloid leukemia), gene analysis, internal tandem duplication (ITD) variants (ie, exons 14, 15)

81246-FLT3 (fms-related tyrosine kinase 3) (eg, acute myeloid leukemia), gene analysis; tyrosine kinase domain (TKD) variants (eg, D835, I836)

LOINC Code Information

Test ID	Test Order Name	Order LOINC Value
FLT	FLT3 Mutation Analysis, V	79210-1

Result ID	Test Result Name	Result LOINC Value
MP009	Specimen Type	31208-2
41935	FLT3 Result	79210-1
19236	Final Diagnosis:	34574-4

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Secondary ID

19739