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HelpTest Code LAB827 Tryptase, Serum
Additional Codes
TRYPT
Useful For
Evaluation of individuals with suspected mast cell activation, which may occur as a result of anaphylaxis or allergen challenge
Evaluation of patients with suspected cutaneous or systemic mastocytosis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Additional Information: Tryptase degenerates very quickly when left in the presence of red blood cells.
Specimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 14 days | |
| Refrigerated | 7 days |
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Send in plastic vial, Frozen
Off-campus collections:
Centrifuge and aliquot within 2 hours.
Specimen to be stored/transported Frozen
Reference Values
<11.5 ng/mL
Day(s) Performed
Monday through Friday
CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
Interpretation
Transient tryptase concentrations greater than or equal to 11.5 ng/mL may be consistent with mast cell activation in the context of anaphylaxis or allergen challenge; measurement of tryptase in specimens obtained 1 to 6 hours and at least 24 hours after the episode may be useful in demonstrating a return to baseline concentrations.
Basal tryptase concentrations greater than or equal to 11.5 mg/mL may be consistent with cutaneous mastocytosis.
Basal tryptase concentrations greater than or equal to 20 ng/mL may be consistent with systemic mastocytosis.
Method Description
Anti-tryptase, covalently coupled to ImmunoCAP, reacts with tryptase in the patient serum specimen. After washing, enzyme-labeled antibodies against tryptase are added to form a complex. After incubation, unbound enzyme-labeled antibodies are washed away, and the bound complex is incubated with a developing agent. After stopping the reaction, the fluorescence in the eluate is measured. The fluorescence is directly proportional to the concentration of tryptase in the serum specimen.(Package insert: ImmunoCAP Tryptase. Phadia AB; 10/2019)
Report Available
2 to 5 daysSpecimen Retention Time
14 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Secondary ID
81608Testing Algorithm
For more information, see Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow