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Test Code LAB7541 Clonazepam and 7-Aminoclonazepam, Serum

Additional Codes

CZPS

Test Down Notes

This test is temporarily unavailable due to instrumentation issues. As an alternate, order FCLMS. For additional details, see test update here.

Useful For

Assessing patient compliance

 

Monitoring for appropriate therapeutic level

 

Assessing clonazepam toxicity

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Specimen Type

Serum Red


Specimen Required


Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.2 mL

Collection Instructions:

1. Draw blood immediately before next scheduled dose (minimum 12 hours after last dose).

2. Within 2 hours of collection, the specimen must be centrifuged and the serum aliquoted into a plastic vial.


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 14 days
  Frozen  28 days
  Ambient  72 hours
Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318.Send serum in plastic vial, Refrigerated.
 
Off-campus collections: 
Centrifuge and aliquot within 2 hours.
Specimen to be stored/transported at Refrigerated temp. 

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus Reject

Reference Values

Clonazepam

Anticonvulsant: 20-70 ng/mL

Anxiolytic: 4-80 ng/mL

Some individuals may show therapeutic response outside of these ranges or may display toxicity within the therapeutic range, thus interpretation should include clinical evaluation. 

Note: Therapeutic ranges are for specimens collected at trough (ie, immediately before next scheduled dose). Levels may be elevated in non-trough specimens.

Interpretation

The therapeutic range varies depending on the indication.

 

Some individuals may respond well outside of these ranges or may display toxicity within the therapeutic range, and thus, interpretation should include clinical evaluation.

 

The possibility of toxicity is increased when levels exceed 100 ng/mL.

Method Description

Clonazepam and 7-aminoclonazepam are extracted from serum using a liquid-liquid extraction technique. The organic layer is removed, dried, and reconstituted. Analysis is by liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)

Day(s) Performed

Monday, Wednesday

Report Available

3 to 7 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CZPS Clonazepam and 7-Aminoclonazepam, S 104541-8

 

Result ID Test Result Name Result LOINC Value
65044 Clonazepam 3494-2
41782 7-Aminoclonazepam 28059-4

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.