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Test Code LAB7510 Coccidioides Antibody Screen with Reflex, Serum

Additional Codes

COXIS

Useful For

Detecting antibodies to Coccidioides immitis/posadasii

 

This assay should not be used for monitoring response to therapy.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RSCOC Coccidioides Ab, CompF/ImmDiff,S Yes, (order SCOC) No

Testing Algorithm

If result is reactive, then Coccidioides by complement fixation and immunodiffusion will be performed at an additional charge.

 

For more information see Meningitis/Encephalitis Panel Algorithm.

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

1.7 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318 
Submission Container/Tube: Plastic via, Refrigerated.
 
Off-campus collections: Centrifuge and aliquot within 2 hours. Specimen to be stored/transported at Refrigerated temp. 

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Negative

 

Reference value applies to all ages

Interpretation

Enzyme immunoassay (EIA) results greater than or equal to 0.75 will be reported as Reactive: Confirmatory testing by complement fixation and immunodiffusion has been ordered.

 

A reactive result is presumptive evidence that the patient was previously or is currently infected with Coccidioides immitis/posadasii.

 

EIA results less than 0.75 will be reported as Negative: Repeat testing on a new sample in 2 to 3 weeks if clinically indicated.

 

A negative result indicates the absence of antibodies to C immitis/posadasii. It is presumptive evidence that the patient has not been previously exposed to, and is not infected with, Coccidioides. However, a negative result does not preclude the diagnosis of coccidioidomycosis as the specimen may have been collected before antibody levels were detectable, due to early acute infection or immunosuppression.

 

This test is designed for the qualitative detection of both IgM- and IgG-class antibodies against antigens from Coccidioides. The report will not indicate which class of antibody is present.

Method Description

Microwells are coated with recombinant Coccidioides complement fixing (CF) and tube precipitin (TP) antigens. Diluted serum specimens and controls are incubated in the wells, and if present, antibodies to TP and CF will bind to the adhered antigen. Nonspecific reactants are removed by washing; peroxidase-conjugated, secondary antihuman antibody is then applied to the wells and incubated. The conjugated secondary antibody will bind to the patient antibodies. Substrate solution is added to the wells, activating the peroxidase conjugate to develop a color reaction. Stop solution is added and the color change is quantified by measuring the optical density.(Package insert: clarus Cocci AB EIA. Immy; Revision 03/06/2020)

Day(s) Performed

Monday through Friday

Report Available

1 to 7 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86635

86635 x3 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
COXIS Coccidioides Ab Screen w/Reflex, S 40712-2

 

Result ID Test Result Name Result LOINC Value
COXQ2 Coccidioides Ab Screen, S 40712-2

Method Name

COXIS: Enzyme Immunoassay (EIA)

RSCOC: Complement Fixation (CF)/Immunodiffusion (ID)

Secondary ID

62079

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.