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Test Code LAB7497 Free Fatty Acids, Total, Serum

Additional Codes

NEFA

Useful For

Evaluation of metabolic status of patients with endocrinopathies

 

Monitoring of control of diabetes mellitus

 

Monitoring the effects of therapeutic diet/exercise lifestyle changes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Patient Preparation:

1. Fasting-overnight (12-14 hours).

2. Patient must not consume any alcohol for 24 hours before the specimen is collected.

3. Patient should not be receiving therapeutic heparin.

Collection Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge within 45 minutes of collection and aliquot 1 mL of serum into a plastic vial.

2. Immediately freeze specimen.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen 7 days
Medical University of South Carolina Note:

On-campus collections: Sent in plastic vial, Frozen.
 
Off-campus collections: Centrifuge and aliquot within 45 minutes. Specimen to be stored/transported at Frozen temp. 
 

Reference Values

≥18 years: 0.00-0.72 mmol/L

Reference values have not been established for patients who are <18 years of age.

Day(s) Performed

Monday through Friday

CPT Code Information

82725

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NEFA Free Fatty Acids, Total, S 15066-4

 

Result ID Test Result Name Result LOINC Value
NEFA Free Fatty Acids, Total, S 15066-4

Interpretation

Abnormally high levels of free fatty acids are associated with uncontrolled diabetes mellitus and with conditions that involve excessive release of a lipoactive hormone such as epinephrine, norepinephrine, glucagon, thyrotropin, and adrenocorticotropin.

Method Description

In the presence of adenosine triphosphate and coenzyme A (CoA), serum non-esterified fatty acids form acyl-CoA, adenosine monophosphate and pyrophosphate when treated with acylCoA synthetase. The acylCoA is then oxidized by adding acylCoA oxidase to produce hydrogen peroxide, which in the presence of added peroxidase allows for the oxidative condensation of 3-methyl-N- ethyl-N-(beta-hydroxyethyl)-aniline with 4-aminoantipyrine to form a purple colored end product.(Package insert: HR Series NEFA-HR(2). Fujifilm Healthcare Americas Corporation; 22.01.20K08 12/2021)

Report Available

1 to 3 days

Specimen Retention Time

7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

Enzymatic Colorimetric

Forms

If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.

Secondary ID

606892