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Test Code LAB7477 Arbovirus Antibody Panel, IgG and IgM, Spinal Fluid

Additional Codes

ABOPC

Profile Information

Test ID Reporting Name Available Separately Always Performed
CAVPC Calif(LaCrosse) Encep Ab Panel, CSF Yes Yes
EEPC East Equine Enceph Ab Panel, CSF Yes Yes
STLPC St. Louis Enceph Ab Panel, CSF Yes Yes
WEEPC West Equine Enceph Ab Panel, CSF Yes Yes

Method Name

Immunofluorescence Assay (IFA)

Specimen Type

CSF


Ordering Guidance


This panel tests for 4 arboviruses; to test for a specific arbovirus, the following tests are individually orderable:

-CAVPC / California Virus (La Crosse) Encephalitis Antibody Panel, IgG and IgM, Spinal Fluid

-EEPC / Eastern Equine Encephalitis Antibody Panel, IgG and IgM, Spinal Fluid

-STLPC / St. Louis Encephalitis Antibody Panel, IgG and IgM, Spinal Fluid

-WEEPC / Western Equine Encephalitis Antibody Panel, IgG and IgM, Spinal Fluid

 

New York State clients: This test is not available for specimens originating in New York.



Specimen Required


Container/Tube: Sterile vial

Preferred: Vial number 1

Acceptable: Any vial

Specimen Volume: 0.7 mL


Specimen Minimum Volume

0.7 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

CALIFORNIA VIRUS (La CROSSE) ENCEPHALITIS ANTIBODY

IgG: <1:1

IgM: <1:1

Reference values apply to all ages.

 

EASTERN EQUINE ENCEPHALITIS ANTIBODY

IgG: <1:1

IgM: <1:1

Reference values apply to all ages.

 

ST. LOUIS ENCEPHALITIS ANTIBODY

IgG: <1:1

IgM: <1:1

Reference values apply to all ages.

 

WESTERN EQUINE ENCEPHALITIS

IgG: <1:1

IgM: <1:1

Reference values apply to all ages.

Interpretation

Detection of organism-specific antibodies in the cerebrospinal fluid (CSF) may suggest central nervous system (CNS) infection. However, these results are unable to distinguish between intrathecal antibodies and serum antibodies introduced into the CSF at the time of lumbar puncture or from a breakdown in the blood-brain barrier. The results should be interpreted with other laboratory and clinical data prior to a diagnosis of CNS infection.

Method Description

The indirect immunofluorescent antibody (IFA) assay is a 2-stage "sandwich" procedure. In the first stage, the patient cerebrospinal fluid (CSF) is diluted in Pretreatment Diluent for IgM and phosphate buffered saline (PBS) for IgG, added to appropriate slide wells in contact with the substrate, and incubated. Following incubation, the slide is washed in PBS, which removes unbound CSF antibodies. In the second stage, each antigen well is overlaid with fluorescein-labeled antibody to IgM and IgG. The slide is incubated allowing antigen-antibody complexes to react with the fluorescein-labeled anti-IgM and anti-IgG. After the slide is washed, dried, and mounted, it is examined using fluorescence microscopy. Positive reactions appear as cells exhibiting bright apple-green cytoplasmic fluorescence against a background of red negative control cells. Semi-quantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(Package inserts: Arbovirus IFA IgM and Arbovirus IFA IgG Instructions for Use. Focus Diagnostics; Rev 03, 02/17/2023)

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 4 days

Specimen Retention Time

2 weeks

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86651 x 2

86652 x 2

86653 x 2

86654 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ABOPC Arbovirus Ab Panel IgG and IgM, CSF 49094-6

 

Result ID Test Result Name Result LOINC Value
26365 Calif(LaCrosse) Encep Ab, IgG,CSF 9539-8
26369 East Equine Enceph Ab, IgG, CSF In Process
26367 St. Louis Enceph Ab, IgG, CSF 21509-5
26371 West Equine Enceph Ab, IgG, CSF 9315-3
26372 West Equine Enceph Ab, IgM, CSF 9316-1
26368 St. Louis Enceph Ab, IgM, CSF 21510-3
26370 East Equine Enceph Ab, IgM, CSF 10899-3
26366 Calif(LaCrosse) Encep Ab, IgM,CSF 9540-6

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Testing Algorithm

The following algorithms are available:

-Meningitis/Encephalitis Panel Algorithm

-Mosquito-borne Disease Laboratory Testing