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Test Code LAB7475 Mumps Virus Antibody, IgM, Serum

Additional Codes

MMPM

Useful For

Laboratory diagnosis of mumps virus infection

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

Serum: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318 
Submission Container/Tube: Plastic vial, Refrigerated
 
Off-campus collections: 
Centrifuge and aliquot within 2 hours.
Specimen to be stored/transported Refrigerated
 

Reference Values

Negative: Index value 0.00-0.79

Reference value applies to all ages.

Day(s) Performed

Tuesday

CPT Code Information

86735

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MMPM Mumps Ab, IgM, S 6478-2

 

Result ID Test Result Name Result LOINC Value
MUMP1 Mumps Ab, IgM, S 6478-2

Interpretation

Positive:

Results suggest recent infection or vaccination.  False positive results may occur in patients with recent parvovirus B19, cytomegalovirus or Epstein-Barr virus infection.

 

Equivocal:

Recommend follow-up testing in 10 to14 days if clinically indicated.

 

Negative:

Absence of IgM-class antibodies to mumps virus suggests lack of acute phase infection with mumps virus. However, serology may be negative in early disease, and results should be interpreted in the context of clinical findings.

Method Description

The ASI Mumps IgM EIA (enzyme immunoassay) is based on the principle of the capture of serum immunoglobulins by anti-human IgM monoclonal antibodies found on the solid phase. A subsequent incubation with mumps antigen in a complex with monoclonal antibodies conjugated to horse radish peroxidase selects the IgM antibodies specific for the antigen. Peroxidase substrate is added and the enzymatic reaction is stopped by the addition of a sulfuric acid solution which results in a yellow color change. The intensity of the yellow color is proportional to the amount of specific antibodies present in the sample and is read in an enzyme-linked immunosorbent assay microplate reader.(Package insert: ASI Mumps IgM EIA test. Arlington Scientific Inc; 860096AM Rev; 03/14/2025)

Report Available

1 to 7 days

Specimen Retention Time

14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Heat-inactivated specimen Reject

Method Name

Enzyme Immunoassay (EIA)

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Secondary ID

80977