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HelpTest Code LAB7470 Lipid Analysis, Body Fluid
Additional Codes
BFLA1
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Specimen Type: Body fluid
Preferred Sources:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, JP drain)
-Pericardial
Acceptable Source:
Collection Container/Tube: Sterile container, no additive
Submission Container/Tube: Plastic vial
Specimen Volume: 3 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Secondary ID
614164Useful For
Distinguishing between chylous and nonchylous effusions
Method Name
Colorimetry/Spectrophotometry (SP)/Electrophoresis
Specimen Type
Body FluidSpecimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Body Fluid | Frozen (preferred) | 30 days | |
| Refrigerated | 7 days | ||
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
| Breast milk Nasal secretions Gastric secretions Bronchoalveolar lavage (BAL or bronchial washings) Colostomy/ostomy Amniotic fluid Feces Saliva Sputum Urine Spinal fluid Synovial fluid Vitreous fluid |
Reject |
Reference Values
An interpretive report will be provided.
Interpretation
Pleural Fluid:
Pleural fluid cholesterol concentrations 46 to 65 mg/dL are consistent with exudative effusions.
Cholesterol concentrations greater than 200 mg/dL suggest pseudochylous effusion.
Triglyceride concentrations greater than 110 mg/dL are consistent with chylous effusions.
Triglyceride concentrations less than 50 mg/dL are usually not due to chylous effusions.
Peritoneal Fluid:
Peritoneal fluid triglyceride concentrations greater than 187 mg/dL are most consistent with chylous effusion.
Cholesterol concentrations 33 to 70 mg/dL suggest malignant causes of ascites.
Method Description
Electrophoresis:
Samples are analyzed for qualitative interpretation of chylomicrons and plasma lipoproteins using a lipid specific Sudan black stain.(Package insert: Hydragel 7, 15 and 30 LIPO + Lp(a). Sebia Inc; 07/2020)
Cholesterol:
Cholesterol esters are cleaved by the action of cholesterol esterase to yield free cholesterol and fatty acids. Cholesterol oxidase then catalyzes the oxidation of cholesterol to cholest-4-en-3-one and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide formed effects the oxidative coupling of phenol and 4-aminophenazone to form a red quinone-imine dye. The color intensity of the dye formed is directly proportional to the cholesterol concentration. It is determined by measuring the increase in absorbance.(Package insert: Cholesterol Gen2 Reagent. Roche Diagnostics; V 16.0, 10/2023)
Triglycerides:
Samples analyzed for triglycerides are measured by an automated enzymatic method. The chemistry includes hydrolysis of the triglycerides and phosphorylation of the resulting glycerol.(Package insert: Triglycerides Reagent. Roche Diagnostics; V13.0, 03/2022)
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysSpecimen Retention Time
3 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82664-Electrophoretic technique, not elsewhere specified (Chylomicrons and lipoproteins)
84311-Spectrophotometry, analyte not specified (Cholesterol)
84478-Triglycerides
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BFLA1 | Lipid Analysis, BF | 94872-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| FLD28 | Fluid Type: | 14725-6 |
| BFCHO | Cholesterol, BF | 12183-0 |
| BFTRG | Triglycerides, BF | 12228-3 |
| BFCMT | Comment | 21025-2 |