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Test Code LAB745 Thiamine (Vitamin B1), Whole Blood

Additional Codes

TDP

B1

Secondary ID

42356

Useful For

Assessment of thiamine deficiency

 

Measuring thiamine levels in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Specimen Type

Whole Blood EDTA


Shipping Instructions


Ship specimen in amber vial or tube to protect from light.



Specimen Required


Patient Preparation:

1. Patient should fast overnight (12-14 hours); infants should have specimen collected just before next feeding. Water can be taken as needed.

2. For 12 hours before specimen collection, patient should not take vitamin supplements.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Amber vial

Specimen Volume: 4 mL

Collection Instructions:

1. Invert 8 to 10 times to mix blood.

2. Transfer whole blood into amber vial or tube and freeze within 24 hours of collection.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Frozen 28 days LIGHT PROTECTED
Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318 
Submission Container/Tube: Amber vial, Frozen
 
Off-campus collections: Protect from light.
DO NOT SPIN! DO NOT SHARE! 
Specimen to be stored/transported Frozen

Reject Due To

Gross lipemia Reject
Glass vial
Clotted specimen		 Reject

Reference Values

70-180 nmol/L

Interpretation

Values for thiamine diphosphate of less than 70 nmol/L are suggestive of thiamine deficiency.

Method Description

Samples are extracted with methanol and an isotopically labeled internal standard. Following centrifugation, an aliquot of the supernatant is dried down and reconstituted. The analyte is then detected using liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

3 to 6 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84425

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TDP Thiamin (Vitamin B1), WB 32554-8

 

Result ID Test Result Name Result LOINC Value
85753 Thiamin (Vitamin B1), WB 32554-8

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.