Home
HelpTest Code LAB580 Vitamin A, Serum
Additional Codes
VITA
Secondary ID
42357Useful For
Diagnosing vitamin A deficiency and toxicity
Monitoring vitamin A therapy
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Specimen Type
SerumSpecimen Required
Patient Preparation: Patient should fast overnight (12-14 hours); infants should have specimen collected before next feeding.
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial. Serum must be removed from the red blood cells within 2 hours.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 28 days | |
| Ambient | 28 days | ||
| Frozen | 28 days |
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Submit in plastic vial, Refrigerated.
Off-campus collections:
Centrifuge and aliquot within 2 hours.
Specimen to be stored/transported at Refrigerated temp.
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
0-6 years: 11.3-64.7 mcg/dL
7-12 years: 12.8-81.2 mcg/dL
13-17 years: 14.4-97.7 mcg/dL
≥18 years: 32.5-78.0 mcg/dL
Interpretation
The World Health Organization recommendations supplementation when vitamin A levels fall below 20.0 mcg/dL.
Severe deficiency is indicated at levels less than 10.0 mcg/dL.
Vitamin A values above 120.0 mcg/dL suggest hypervitaminosis A and associated toxicity.
Method Description
Deuterated vitamin A (d6-all-trans retinol) is added to serum as an internal standard. Vitamin A (all-trans retinol) and the deuterated internal standard are extracted from the specimens using on-line turbulent flow high-performance liquid chromagraphy and analyzed by liquid chromatography-tandem mass spectrometry using multiple reaction monitoring in positive mode.(Unpublished Mayo method)
Day(s) Performed
Sunday through Friday
Report Available
2 to 5 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84590
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VITA | Vitamin A, S | 2923-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 7597 | Vitamin A | 2923-1 |
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.