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Test Code LAB536 1,25-Dihydroxyvitamin D, Serum

Additional Codes

DHVD

D125

VITAMIN D125

Useful For

As a second-order test in the assessment of vitamin D status, especially in patients with renal disease

 

Investigation of some patients with clinical evidence of vitamin D deficiency (eg, vitamin D-dependent rickets due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxyvitamin D)

 

Differential diagnosis of hypercalcemia

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Ordering Guidance


The 25-hydroxyvitamin D test (25HDN / 25-Hydroxyvitamin D2 and D3, Serum) in serum is the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores. In the presence of renal disease or hypercalcemia, testing of 1,25-dihydroxy vitamin D (DHVD) may be needed to adequately assess vitamin D status.



Specimen Required


Patient Preparation: Fasting is preferred for 4 hours but not required.

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.7 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days
  Ambient  7 days

Reference Values

Males:

<16 years: 24-86 pg/mL

≥16 years: 18-64 pg/mL

 

Females:

<16 years: 24-86 pg/mL

≥16 years: 18-78 pg/mL

 

For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) Performed

Monday through Friday

CPT Code Information

82652

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DHVD 1,25-Dihydroxyvitamin D, S 62290-2

 

Result ID Test Result Name Result LOINC Value
8822 1,25-Dihydroxyvitamin D, S 62290-2

Interpretation

1,25-Dihydroxyvitamin D (DVHD) concentrations are low in chronic renal failure and hypoparathyroidism.

 

1,25-Dihydroxyvitamin D concentrations are high in sarcoidosis and other granulomatous diseases, some malignancies, primary hyperparathyroidism, and physiologic hyperparathyroidism.

 

1,25-Dihydroxyvitamin D concentrations are not a reliable indicator of vitamin D toxicity; normal (or even low) results may be seen in such cases.

Method Description

Deuterated stable isotopes are added to sample as internal standard. 1,25-Dihydroxyvitamin D and the internal standard are extracted. The extracts are then further purified by solid phase extraction (SPE) and affinity extraction. Extracts are then derivatized and analyzed by chromatography-tandem mass spectrometry using multiple reaction monitoring.(Unpublished Mayo method)

Report Available

2 to 5 days

Specimen Retention Time

2 weeks

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Renal Diagnostics Test Request (T830)