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Test Code LAB3023 Strongyloides Antibody, IgG, Serum

Additional Codes

STRNG

Useful For

Screening for the presence of IgG-class antibodies to Strongyloides

 

This test is not useful for monitoring patient response to therapy as IgG-class antibodies to Strongyloides may remain detectable following resolution of infection.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days
Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318 
Submission Container/Tube: Plastic vial, Refrigerated
 
Off-campus collections: 
Centrifuge and aliquot within 2 hours.
Specimen to be stored/transported Refrigerated

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Interpretation

Positive:

IgG antibodies to Strongyloides were detected, suggesting current or past infection. False-positive results may occur with other helminth infections (eg, Trichinella, Taenia solium). Clinical correlation is required.

 

Negative:

No detectable levels of IgG antibodies to Strongyloides. Repeat testing in 10 to 14 days if clinically indicated.

Method Description

The Bordier Strongyloides ratti IgG enzyme-linked immunosorbent assay is an enzymatically amplified sandwich-type immunoassay. After a blocking step, diluted serum and controls are incubated in antigen-coated microtiter wells, then washed and incubated with antihuman-IgG antibody labeled with protein A-alkaline phosphatase conjugate. After a washing step, the wells are incubated with phosphatase substrate. The reaction is halted by potassium phosphate stopping solution and the degree of enzymatic turnover is determined by absorbance measured at 405 nanometers with a reference filter of 590 to 650 nm.(Package insert: Strongyloides Ratti Enzyme immunoassay for the diagnosis of human Strongyloidosis. Bordier Affinity Products SA; 01/2018)

Day(s) Performed

Monday, Wednesday, Thursday, Friday

Report Available

Same day/1 to 4 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86682

LOINC Code Information

Test ID Test Order Name Order LOINC Value
STRNG Strongyloides Ab, IgG, S 34376-4

 

Result ID Test Result Name Result LOINC Value
STRNG Strongyloides Ab, IgG, S 80660-4

Testing Algorithm

For more information see Parasitic Investigation of Stool Specimens Algorithm.

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.

Secondary ID

63866