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HelpTest Code LAB2616 Platelet Antibody Screen, Serum
Additional Codes
PLABN
Useful For
Detecting alloantibodies to epitopes on platelet glycoproteins IIb/IIIa, Ib/Ix, Ia/IIa, IV and class I human leukocyte antigens to evaluate cases of immune mediated refractoriness to platelet transfusions, posttransfusion purpura, or neonatal alloimmune thrombocytopenia
Special Instructions
Method Name
Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)
Specimen Type
Serum RedOrdering Guidance
For neonate testing, consider sending a maternal specimen instead of a neonate specimen as unbound platelet antibodies may not be detected in the neonate serum.
This test is not recommended for the diagnosis of immune thrombocytopenia or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.
Necessary Information
If ordering electronically, answer all prompt questions for timely result reporting:
1. Reason for request is required for result interpretation.
a. Use provided diagnosis options if appropriate. If specific diagnosis is unknown, select the generic answer of alloimmune thrombocytopenia.
b. Record only the diagnosis pertaining to this test.
c. Record diagnosis description instead of code.
2. Indicate if patient has had intravenous immunoglobulin (IVIg) therapy in the last month: Yes or No
Specimen Required
Patient Preparation: Do not collect within 72 hours of a platelet transfusion. Transfused platelets will interfere with this assay.
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Frozen (preferred) | 365 days | |
| Refrigerated | 48 hours |
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Send serum in plastic vial, Frozen
Off-campus collections: Centrifuge and aliquot within 2 hours. Specimen to be stored/transported at Frozen temp.
Reject Due To
| Gross hemolysis | Reject |
Reference Values
Not applicable
Method Description
Patient serum is added to microwells coated with platelet and human leukocyte antigen (HLA) glycoproteins, allowing antibody, if present, to bind. Unbound antibodies are then washed away. An alkaline phosphatase-labeled antihuman globulin reagent (anti-IgG/A/M) is added to the microwells and incubated. The unbound anti-IgG/A/M is washed away and the substrate p-nitrophenylphosphate (PNPP) is added to the wells and incubated. The reaction is stopped with stopping solution. The optical density of the color that develops is measured in a spectrophotometer and results are interpreted.(Package insert: PakPlus. Immucor GTI Diagnostics; 303469.IFUEN Rev E; 07/2015)
Day(s) Performed
Monday through Friday
Report Available
2 to 4 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86022
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PLABN | Platelet Ab Screen, S | 95270-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| PTL01 | Overall Result | 24375-8 |
| PTL02 | Interpretation | 59466-3 |
| PTL03 | GPIIb/IIIa (Cell-1) | 48505-2 |
| PTL04 | GPIIb/IIIa (Cell-2) | 48505-2 |
| PTL05 | GPIa/IIa (Cell-1) | 47084-9 |
| PTL06 | GPIa/IIa (Cell-2) | 47084-9 |
| PTL07 | GPIb/IX | 48506-0 |
| PTL08 | GPIV | 87757-1 |
| PTL09 | HLA Class I | 95269-7 |
| PTL15 | Reason for request? | 29548-5 |
| PTL16 | IVIg in last month? | 95268-9 |
Testing Algorithm
For more information see Platelet Antibody Testing Algorithm.
Secondary ID
35794Interpretation
This assay screens patient sera for platelet-reactive antibodies via enzyme-linked immunosorbent assay.