Anaerobic Culture
Additional Codes:
C ANA
Collection:
SPECIMEN:
- Abscess or Wound (specify source/type): Collect from margin of leading edge of wound.
- CSF or other CNS fluids. Specify if CSF is shunt fluid on request card.
- Cornea or Corneal Lesions: Inoculate directly to media.
- Aqueous or Vitreous Humor: Send capped syringe (without needle) or inoculate directly to media.
- Sterile body fluid (specify source/type): Submit sterile container or capped syringe (without needle).
NOTE: If submitted in blood culture bottles, order LAB2101 Sterile Body Fluids culture.
- Tissue or Organ Biopsy (specify source/type): Submit sterile container.
CONTAINER / VOLUME:
- Abscess or Wound: 1 mL minimum - sterile container (aspirate preferable) or 2 E-swabs.
- CSF: 1 mL, Minimum 0.5 mL, sterile container or CSF Tube (usually #3)
- Sterile Body Fluids: 5 mL; Minimum 1 mL, sterile container
- Tissue or Organ Biopsy: 1-gram minimum, sterile container
UNACCEPTABLE/REJECTED SPECIMENS:
Sources where anaerobes are part of normal flora, including
- Respiratory Specimens, i.e. sputum, endotracheal, tracheal aspirate, bronchial brushings, BAL secretions, washings.
- Genital specimens, i.e. vaginal, cervical, urethral, vulval, locial, endocervical.
- Intestinal specimens, i.e. stool
Catheter IV tips, Foley and other bladder catheter tips, Urine.
Storage/Transport Temperature:
Sample is stable 2 hours ambient; and up to 48 hours ambient in anaerobic or Eswab transport media.
Includes
If pericardial, pleural, peritoneal or joint fluids are being submitted in blood culture bottles, order LAB2101 Sterile Body Fluids culture.
Turnaround Time (TAT):
After 48 hours for preliminary positive cultures. Negative reports after 5 days.
Test Availability:
24 hours a day / 7 Days a week
Request Form:
Microbiology – Green Request Form
Send To:
UMC MAIN LABORATORY Specimen Receiving – EH318 Children’s Hospital
Reference Interval:
No anaerobic organisms isolated.