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Anaerobic Culture

Important Note

Please order LAB2837 Bacterial Culture Routine.  Anaerobe culture is automatically reflex ordered for appropriate sources

Additional Codes:

C ANA

Collection:

SPECIMEN:

- Abscess or Wound (specify source/type):  Collect from margin of leading edge of wound.

- CSF or other CNS fluids.  Specify if CSF is shunt fluid on request card.

- Cornea or Corneal Lesions:  Inoculate directly to media.

- Aqueous or Vitreous Humor:  Send capped syringe (without needle) or inoculate directly to media.

- Sterile body fluid (specify source/type):  Submit sterile container or capped syringe (without needle). 

     NOTE:  If submitted in blood culture bottles, order LAB2101 Sterile Body Fluids culture.

- Tissue or Organ Biopsy (specify source/type):  Submit sterile container.

 

CONTAINER / VOLUME:

- Abscess or Wound:  1 mL minimum - sterile container (aspirate preferable) or 2 E-swabs.

- CSF:  1 mL, Minimum 0.5 mL, sterile container or CSF Tube (usually #3)

- Sterile Body Fluids:  5 mL; Minimum 1 mL, sterile container

- Tissue or Organ Biopsy:  1-gram minimum, sterile container

 

UNACCEPTABLE/REJECTED SPECIMENS: 

Sources where anaerobes are part of normal flora, including

- Respiratory Specimens, i.e. sputum, endotracheal, tracheal aspirate, bronchial brushings, BAL secretions, washings.

- Genital specimens, i.e. vaginal, cervical, urethral, vulval, locial, endocervical.

- Intestinal specimens, i.e. stool

Catheter IV tips, Foley and other bladder catheter tips, Urine.

Storage/Transport Temperature:

Sample is stable 2 hours ambient; and up to 48 hours ambient in anaerobic or Eswab transport media.

Includes

If pericardial, pleural, peritoneal or joint fluids are being submitted in blood culture bottles, order LAB2101 Sterile Body Fluids culture. 

Turnaround Time (TAT):

After 48 hours for preliminary positive cultures. Negative reports after 5 days.

Test Availability:

24 hours a day / 7 Days a week

Request Form:

Microbiology – Green Request Form

Send To:

UMC MAIN LABORATORY Specimen Receiving – EH318 Children’s Hospital

Reference Interval:

No anaerobic organisms isolated.