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HelpTest Code LAB2254 Mitochondrial Antibodies (M2), Serum
Additional Codes
AMA
Useful For
Establishing the diagnosis of primary biliary cholangitis
This test is not useful for indicating the stage or prognosis of the disease or for monitoring the course of the disease.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318. Send in plastic vial, Refrigerated.
Off-campus collections: Centrifuge and aliquot within 2 hours. Specimen to be stored/transported Refrigerated
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reference Values
Negative: <0.1 Units
Borderline: 0.1-0.3 Units
Weakly positive: 0.4-0.9 Units
Positive: ≥1.0 Units
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
CPT Code Information
86381
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AMA | Mitochondrial Ab, M2, S | 51715-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| AMA | Mitochondrial Ab, M2, S | 51715-1 |
Interpretation
A positive result for antimitochondrial antibodies of M2 specificity in the setting of chronic cholestasis after exclusion of other causes of liver disease is highly suggestive of primary biliary cholangitis.
Method Description
A recombinant pyruvate dehydrogenase complex-E2 (M2) antigen for detection of antibodies against M2 is attached to the surface of a microplate. Diluted patient serum, standards, or controls are added to the wells, and the M2 specific IgG and IgM antibodies, if present, bind to the antigen. All unbound human antibodies are washed away, and a conjugate of enzyme-labeled polyclonal antibody to human IgG and IgM is added. The enzyme conjugate binds to the antibody complex. Excess enzyme-conjugate is washed away, and substrate is added. After a specified time, the enzyme reaction is stopped. The intensity of the color generated is proportional to the amount of anti-M2 IgG and/or IgM antibody in the sample. The results are read by a spectrophotometer producing a direct measurement of the anti-M2 IgG and IgM antibodies in the serum. Testing is performed on the Agility instrument by Dynex.(Package insert: Kallestad Anti-Mitochondrial Kit. Bio-Rad Laboratories, Inc; 10/2014)
Report Available
2 to 3 daysSpecimen Retention Time
14 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimens | Reject |
Method Name
Enzyme Immunoassay (EIA)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)