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Test Code LAB1933 Amylase, Isoenzymes, Serum

Additional Codes

AMISO


Necessary Information


Age and sex of patient are required.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.


Medical University of South Carolina Note:

On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318.  Send in plastic vial, Refrigerated

 

Off-campus collections: Centrifuge and aliquot within 2 hours. Specimen to be stored/transported at Refrigerated temp.

Secondary ID

604930

Useful For

Ruling out salivary amylase as the cause of elevated serum amylase

Profile Information

Test ID Reporting Name Available Separately Always Performed
AMYSE Amylase, Total, S Yes, (Order AMS) Yes
AMYPA Amylase, Pancreatic, S No Yes
AMYSA Amylase, Salivary, S No Yes

Testing Algorithm

Total and pancreatic amylase are measured in the submitted serum specimen. Salivary amylase is calculated as the difference between the two measured results (salivary amylase = total amylase-pancreatic amylase).

Method Name

AMYSE, AMYPA: Colorimetric Rate Reaction

AMYSA: Calculation

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days
  Ambient  7 days

Reject Due To

Gross hemolysis Reject

Reference Values

AMYLASE, TOTAL

0-30 days: ≤6 U/L

31-182 days: 1-17 U/L

183-365 days: 6-44 U/L

1-3 years: 8-79 U/L

4-17 years: 21-110 U/L

≥18 years: 28-100 U/L

 

AMYLASE, PANCREATIC

0-<24 months: ≤20 U/L

2-<18 years: 9-35 U/L

≥18 years: 13-53 U/L

 

AMYLASE, SALIVARY

0-<18 years: Not established

≥18 years: ≤86 U/L

Interpretation

Increased concentrations of total amylase activity in conjunction with increased concentration of specific amylase isoenzymes may aid in differentiating the source of amylase (pancreatic versus salivary).

Method Description

Total amylase:

The liquid Roche amylase method is an enzymatic colorimetric test using 4,6-ethylidene-(G7) p-nitrophenyl-(G1)-alpha-D-maltoheptaoside (ethylidene-G7PNP), cleaved under the catalytic action of alpha-amylases. The G2PNP, G3PNP, and G4PNP fragments formed are completely hydrolyzed to p-nitrophenol and glucose by alpha-glucosidase. The color intensity of the p-nitrophenol formed is directly proportional to the alpha-amylase activity. It is determined by measuring the increase in absorbance.(Package insert: AMYL2 reagent. Roche Diagnostics; 12/2018)

 

Pancreatic amylase:

After immunoinhibition with antibodies against human salivary alpha-amylase, the amount of pancreatic alpha-amylase in a sample is selectively determined by an enzymatic colorimetric method using the substrate ethylidene-G7PNP.(Package insert: Alpha-Amylase EPS Pancreatic. Roche Diagnostics; 05/2019)

Day(s) Performed

Monday through Sunday

Report Available

1 to 3 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82150 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AMISO Amylase, Isoenzymes, S 24333-7

 

Result ID Test Result Name Result LOINC Value
AMYPA Amylase, Pancreatic, S 1805-1
AMYSA Amylase, Salivary, S 1809-3
AMYSE Amylase, Total, S 1798-8

Forms

If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen.