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HelpTest Code LAB1176 Granulocyte Antibodies, Serum
Additional Codes
LAGGT
Useful For
Work-up of individuals having febrile, nonhemolytic transfusion reactions
Detection of individuals with autoimmune neutropenia
This test is not useful for the diagnosis of neutropenia caused by marrow suppression by drugs or tumors.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedSpecimen Required
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Additional Information: Only pretransfusion reaction specimen is acceptable.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 30 days | |
| Frozen | 365 days | ||
| Ambient | 7 days |
Reference Values
Not applicable
Day(s) Performed
Tuesday, Wednesday, Friday
CPT Code Information
86021
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LAGGT | Granulocyte Ab, S | 35279-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LAGG | Granulocyte Ab, S | 35279-9 |
Interpretation
A positive result in an individual being worked up for a febrile transfusion reaction indicates the need for leukocyte-poor (filtered) red blood cells.
This test cannot distinguish between allo- and autoantibodies
Method Description
Purified granulocyte preparations from normal donors are incubated with patient's test serum and then with fluorescein-tagged antihuman globulin reagent. Sera containing the antibodies deposit immunoglobulin on the target cell membrane, which is detected by the second stage antibody and visualized by fluorescence microscopy.(Verheugt FW, von dem Borne AE, Decary F, Engelfreit CP. The detection of granulocyte alloantibodies with an indirect immunofluorescence test. Br J Haematol. 1997;36(4):533-534)
Report Available
7 to 15 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Indirect Immunofluorescence