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HelpTest Code LAB1107 Vasoactive Intestinal Polypeptide, Plasma
Additional Codes
VIP
Useful For
Detecting vasoactive intestinal polypeptide-producing tumors in patients with chronic diarrheal diseases
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Plasma EDTAOrdering Guidance
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial. Freeze immediately.
Specimen Minimum Volume
0.55 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 90 days |
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Centrifuge and Freeze immediately. Submit frozen plasma in plastic vial.
Off-campus collections:
Centrifuge and aliquot within 1 hour.
Specimen to be stored/transported at Frozen temp.
Reference Values
<86 pg/mL
Day(s) Performed
Monday, Thursday
CPT Code Information
84586
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VIP | Vasoactive Intestinal Polypeptide,P | 3125-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8150 | Vasoactive Intestinal Polypeptide,P | 3125-2 |
Interpretation
An elevated vasoactive intestinal polypeptide (VIP) may indicate the presence of an enteropancreatic tumor causing hypersecretion of VIP.
Vasoactive intestinal polypeptide-producing tumors are unlikely with a 24-hour stool volume below 700 mL.
Method Description
Vasoactive intestinal polypeptide (VIP) enzyme-linked immunosorbent assay is a two-step competitive immunoassay. VIP in the patient sample competes with a biotin-labelled antigen for a limited number of anti-VIP antibody binding sites on the microplate wells during the first incubation. After a wash step to remove unbound and excess material the samples are incubated with a streptavidin anti horseradish peroxidase conjugate to form a biotin conjugate complex. After washing, an enzyme substrate is added and incubated. Following the incubation, the reaction is stopped by addition of a stopping solution. Antibody-analyte complex is detected by absorbance measurement at 450 nm wavelength. The absorbance measured is inversely proportional to the concentration of VIP in the samples and calibrators.(Unpublished Mayo method)
Report Available
2 to 4 daysSpecimen Retention Time
14 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.