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HelpTest Code LAB105 Myoglobin, Serum
Additional Codes
MYGLS
Useful For
Assessing muscle damage from any cause
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 365 days | ||
| Ambient | 7 days |
On-campus collections: Tube to 99 or deliver to University Extension Hospital Room EH318
Send Refrigerated
Off-campus collections: Centrifuge and aliquot within 2 hours. Specimen to be stored/transported Refrigerated.
Reference Values
Males: 0 to 72 mcg/L
Females: 0 to 58 mcg/L
Day(s) Performed
Monday through Sunday
CPT Code Information
83874
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MYGLS | Myoglobin, S | 2639-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MYGLS | Myoglobin, S | 2639-3 |
Interpretation
Elevated myoglobin levels are seen in conditions of acute muscle injury.
Method Description
This myoglobin test is a sandwich-principal assay. The first incubation is 9 mcL of sample, a biotinylated monoclonal myoglobin-specific antibody, and a monoclonal myoglobin-specific antibody labeled with a ruthenium complex, which react to form a sandwich complex. In the second incubation, the complex becomes bound to the solid phase via interaction of biotin and streptavidin after addition of streptavidin-coated microparticles. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Results are determined via a calibration curve, which is instrument specifically generated by 2-point calibration, and a master curve provided via the cobas link.(Package insert: Elecsys Myoglobin. Roche Diagnostics; 10/2022)
Report Available
1 to 2 daysSpecimen Retention Time
7 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Method Name
Electrochemiluminescent Immunoassay (ECLIA)