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Test Code IRON Iron, Serum


Specimen Required


Only orderable as part of profile. For more information see SFEC / Iron and Total Iron-Binding Capacity, Serum

 

Patient Preparation:

1. Fasting (12 hours)

2. For 24 hours before collection, patient should not take iron-containing supplements.

 

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Draw blood before noon (preferred).

2. Within 2 hours of collection, serum gel tubes should be centrifuged.

3. Within 2 hours of collection, red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial.


Secondary ID

2502

Useful For

Aiding in the evaluation of iron deficiency and iron overload diseases in combination with total iron binding capacity and percent saturation

 

Assessment of acute iron poisoning

Method Name

Only orderable as part of profile. For more information see SFEC / Iron and Total Iron-Binding Capacity, Serum

 

Colorimetric Assay

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  365 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Reference Values

Only orderable as part of profile. For more information see SFEC / Iron and Total Iron-Binding Capacity, Serum

 

Males: 50-150 mcg/dL

Females: 35-145 mcg/dL

Interpretation

Serum iron is elevated in iron overload conditions including hemochromatosis. Additional causes include oral or parenteral intake of medicinal iron, acute hepatitis, and chronic liver failure.(1)

 

Serum iron is decreased in iron deficiency, iron deficiency anemia, and anemia of chronic disease.(1)

Method Description

Under acidic conditions, iron is liberated from transferrin. Lipemic samples are clarified by the detergent. Ascorbate reduces the released Fe(3+) ions to Fe(2+) ions which then react with FerroZine to form a colored complex. The color intensity is directly proportional to the iron concentration and can be measured photometrically.(Package insert: IRON2. Roche Diagnostics; V9.0, 09/2019)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83540-Iron

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IRON Iron 2498-4

 

Result ID Test Result Name Result LOINC Value
IRON Iron 2498-4