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Test Code IL6DX Interleukin-6, Serum


Specimen Required


Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial


Secondary ID

618776

Useful For

Evaluating patients with suspected chronic inflammatory disorders, such as rheumatoid arthritis, systemic lupus erythematosus, ankylosing spondylitis, or inflammatory bowel disease

 

Evaluating patients with Castleman disease

 

Evaluating patients with suspected systemic infection

 

Evaluating patients with suspected localized infection, specifically prosthetic joint infection

 

Assisting in identifying severe inflammatory response in patients with confirmed COVID-19 illness to aid in determining the risk of intubation with mechanical ventilation, in conjunction with clinical findings and the results of other laboratory testing

Method Name

Immunoenzymatic

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  90 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

< 6.4 pg/mL

Interpretation

Elevated concentrations of interleukin 6 may indicate an ongoing inflammatory response and could be consistent with a systemic infection, localized infection, or chronic inflammatory disease.

Method Description

The Access Interleukin-6 (IL-6) assay is a simultaneous one-step immunoenzymatic (sandwich) assay. A patient sample is added to a reaction vessel along with the paramagnetic particles coated with mouse monoclonal anti-human IL-6, blocking reagent, and the alkaline phosphatase conjugate.

 

After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the chemiluminescent substrate is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of IL-6 in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve.(Instruction manual: Access IL-6 Instructions for Use, Beckman Coulter, Inc; 10/2020)

Day(s) Performed

Monday through Saturday

Report Available

1 to 3 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83529

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IL6DX Interleukin-6, S 26881-3

 

Result ID Test Result Name Result LOINC Value
IL6DX Interleukin-6, S 26881-3