Sign in →

Test Code HISER Histoplasma Antibody Complement Fixation and Immunodiffusion, Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Secondary ID

621213

Useful For

Aiding in the diagnosis of active histoplasmosis

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm.

Method Name

Complement Fixation (CF)/Immunodiffusion (ID)

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Anti-Yeast Antibody by Complement Fixation:

Negative (positive results reported as titer)

 

Antibody by Immunodiffusion:

Negative (positive results reported as band present)

Interpretation

Complement fixation (CF) titer results of 1:32 or higher indicate active disease. A rising CF titer is associated with progressive infection.

 

Patients infected with Histoplasma capsulatum demonstrate a serum antibody with a rising titer within 6 weeks of infection. A rising titer is associated with progressive infection. Specific antibody persists for a few weeks to a year, regardless of clinical improvement.

 

The presence of H and/or M bands on immunodiffusion tests is considered a positive result for the presence of antibodies to Histoplasma. Presence of an H band suggests recent infection.

Method Description

Both immunodiffusion and complement fixation (CF) tests are used to detect antibodies to Histoplasma capsulatum. For immunodiffusion, the antigen used is a culture filtrate. Histoplasmin H and M precipitins can be identified by the assay. For the CF test, antigens are histoplasmin and a yeast form antigen of Histoplasma capsulatum; the latter is more sensitive.(Roberts GD. Fungi. In: Washington II JA, ed. Laboratory Procedures in Clinical Microbiology. 2nd ed. Springer-Verlag, 1985; Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020)

Day(s) Performed

Monday through Friday

Report Available

2 to 7 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86698 x2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HISER Histoplasma Ab CompFix/ImmDiff, S 90227-0

 

Result ID Test Result Name Result LOINC Value
621214 Histoplasma Yeast CompFix, S 20574-0
621215 Histoplasma Immunodiffusion, S 90232-0