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Test Code GMCSF Granulocyte Monocyte-Colony Stimulating Factor, Plasma


Ordering Guidance


If this test is ordered with CYPAN / Cytokine Panel, Plasma, this test will be canceled as duplicate and CYPAN performed as ordered.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Immediately after specimen collection, place tube on wet ice.

2. Centrifuge at 4° C, 1500 x g for 10 minutes.

3. Aliquot plasma into plastic vial.

4. Freeze specimen within 2 hours of collection.


Secondary ID

618775

Useful For

Measuring the concentration of granulocyte macrophage-colony stimulating factor (GM-CSF) in plasma

 

Understanding the etiology of chronic inflammatory diseases or infections, when used in conjunction with clinical information and other laboratory testing

 

Research studies in which an assessment of the GM-CSF response is needed

Method Name

Bead-Based Multiplex Immunoassay

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen 21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-treated specimen Reject

Reference Values

<15.0 pg/mL

Interpretation

Elevated granulocyte macrophage-colony stimulating factor (GM-CSF) concentrations could be consistent with the presence of an inflammatory process or infection.

Method Description

Analyte-specific antibodies are precoated onto color-coded magnetic microparticles. Samples are diluted 1:2 in a mixing plate. Then standards, samples, and microparticles are pipetted into wells, and the immobilized antibodies capture the analytes of interest. Unbound substances are washed away while the magnetic microparticles are immobilized. Next, a biotinylated analyte specific antibody cocktail is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), is added to each well. After removal of unbound Streptavidin-PE and resuspension of the microparticles in buffer, the plate is analyzed using a Luminex FLEXMAP 3D analyzer. A charged-coupled device camera captures an image of each well and data reduction is performed using the XPONENT software.(Unpublished Mayo method)

Day(s) Performed

Wednesday

Report Available

2 to 8 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83520

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GMCSF GM-CSF, P 97054-1

 

Result ID Test Result Name Result LOINC Value
618775 GM-CSF 97054-1