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HelpTest Code EBVAB Epstein-Barr Virus Antibody Profile, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
621373Useful For
Diagnosis of Epstein-Barr virus (EBV) infectious mononucleosis or other EBV related infections
Identification of prior EBV infection as part of pre-immunosuppression screening
This assay is not intended for viral isolation or identification.
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MEBV | EBV VCA IgM, S | No | Yes |
| GEBV | EBV VCA IgG, S | No | Yes |
| NAEBV | EBV NA IgG, S | No | Yes |
| INTEB | Interpretation | No | Yes |
Method Name
MEBV, GEBV, NAEBV: Enzyme-Linked Immunosorbent Assay (ELISA)
INTEB: Technical Interpretation
Specimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 14 days | |
| Refrigerated | 48 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
Epstein-Barr Virus VIRAL CAPSID ANTIGEN (VCA) IgM ANTIBODY:
Negative
Epstein-Barr Virus VIRAL CAPSID ANTIGEN (VCA) IgG ANTIBODY:
Negative
EPSTEIN-BARR VIRUS NUCLEAR ANTIGEN (EBNA) IgG ANTIBODY:
Negative
Interpretation
The profile has 3 components: viral capsid antigen (VCA) IgG, VCA IgM, and Epstein-Barr nuclear antigen (EBNA).
Presence of VCA IgM antibodies suggests an acute or recent primary infection with Epstein-Barr virus (EBV).
Presence of VCA IgG antibodies indicates infection sometime in the recent or remote past.
Antibodies to EBNA develop 6 to 8 weeks after primary infection and are detectable for life.
Refer to table below for interpretation of EBV antibody results.
|
VCA IgM result |
VCA IgG result |
EBNA IgG result |
Interpretation |
|
Negative |
Positive |
Negative |
Results suggest recent EBV infection. The detection of only anti-VCA IgG should be interpreted with caution in immunocompromised patients, as this population may demonstrate diminishing or undetectable levels of anti-EBNA IgG antibodies. |
|
Positive |
Positive |
Negative |
Results suggest recent EBV infection. |
|
Positive |
Negative |
Negative |
|
|
Positive |
Equivocal |
Equivocal |
Results suggest recent EBV infection. Recommend follow-up testing in 10-14 days if clinically indicated. |
|
Positive |
Equivocal |
Negative |
|
|
Positive |
Positive |
Equivocal |
|
|
Positive |
Negative |
Equivocal |
|
|
Positive |
Positive |
Positive |
Results may suggest recent EBV recovery or reactivation. |
|
Positive |
Equivocal |
Positive |
|
|
Positive |
Negative |
Positive |
|
|
Negative |
Negative |
Negative |
Results suggest no prior exposure to EBV. However, a second serum specimen should be tested in 10-14 days if clinically indicated. |
|
Negative |
Positive |
Positive |
Results suggest past EBV infection. |
|
Negative |
Negative |
Positive |
|
|
Negative |
Equivocal |
Positive |
|
|
Negative |
Positive |
Equivocal |
Detection of anti-VCA IgG only should be interpreted with caution in immunocompromised patients, as this population may demonstrate diminishing or undetectable levels of anti-EBNA IgG antibodies. Recommend follow-up testing in 10-14 days if clinically indicated. |
|
Equivocal |
Negative |
Positive |
Results suggest past EBV infection. Recommend follow-up testing in 10-14 days if clinically indicated. |
|
Equivocal |
Equivocal |
Positive |
|
|
|
|
|
Results with unclear clinical significance |
|
Negative |
Negative |
Equivocal |
Recommend follow-up testing in 10-14 days if clinically indicated. |
|
Negative |
Equivocal |
Negative |
|
|
Negative |
Equivocal |
Equivocal |
|
|
Equivocal |
Negative |
Negative |
|
|
Equivocal |
Negative |
Equivocal |
|
|
Equivocal |
Equivocal |
Negative |
|
|
Equivocal |
Equivocal |
Equivocal |
|
|
Equivocal |
Positive |
Negative |
|
|
Equivocal |
Positive |
Equivocal |
|
|
Equivocal |
Positive |
Positive |
Method Description
The ZEUS ELISA EBV (Epstein Barr virus) VCA (viral capture antigen) IgM, VCA IgG, and EBNA-1 (Epstein Barr nuclear antigen-1) IgG Test Systems are designed to detect IgM, IgG, IgG class antibodies to EBNA in human sera. Creation of the sensitized wells of the plastic microwell strips occurred using passive adsorption with EBV antigen. The test procedure involves three incubation steps. Test sera are diluted with the Sample Diluent provided. The IgM Sample Diluent contains antihuman IgG that precipitates and removes IgG and rheumatoid factor from the sample leaving IgM free to react with the immobilized antigen. During sample incubation any antigen specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. Peroxidase Conjugated goat ant-human IgM or IgG is added to the wells and the plate is incubated. The Conjugate will react with IgM or IgG antibody immobilized on the solid phase. The wells are washed to remove unbound Conjugate. The microwells containing immobilized peroxidase Conjugate are incubated with peroxidase Substrate Solution. Hydrolysis of the Substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample.(Package inserts: EBV-VCA IgM Test System. Zeus Scientific, Inc.; 12/13/2017; EBV-VCA IgG Test System. Zeus Scientific, Inc.; 12/19/2017; EBNA-1 IgG Test System. Zeus Scientific, Inc.; 12/19/2017)
Day(s) Performed
Monday through Friday
Report Available
Same day/1 to 2 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86664-EBNA
86665 x 2-VCA, IgG and IgM
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EBVAB | EBV Ab Profile, S | 87554-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| GEBV | EBV VCA IgG, S | 24114-1 |
| INTEB | Interpretation | 69048-7 |
| MEBV | EBV VCA IgM, S | 24115-8 |
| NAEBV | EBV NA IgG, S | 5156-5 |