Sign in →

Test Code CYSTG Cysticercosis (Taenia solium), IgG, Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Secondary ID

616730

Useful For

Aid for the diagnosis of cysticercosis caused by infection with Taenia solium

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Interpretation

Positive:

Antibodies to Taenia solium (cysticercosis) detected. Confirmatory testing through the Centers for Disease Control and Prevention is recommended. False-positive results may occur in patients with other helminth infections.

 

Negative:

No antibodies to T solium (cysticercosis) detected. A negative result may not rule-out infection as the sample may have been collected prior to the development of a detectable level of antibodies. Sensitivity is negatively impacted by the presence of few cysticerci or location in areas less accessible to the immune system. Repeat testing on a new sample is recommended for patients at high risk of cysticercosis.

Method Description

The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Taenia solium cyst soluble antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a protein A - alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of p-nitrophenyl phosphate (pNPP) substrate, which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of T solium specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.(Package insert: Taenia solium Enzyme immunoassay for the diagnosis of human cysticercosis. Bordier Affinity Products SA; 04/2018)

Day(s) Performed

Tuesday

Report Available

1 to 7 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86682

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CYSTG Cysticercosis (T. solium), IgG, S 56485-6

 

Result ID Test Result Name Result LOINC Value
CYSTG Cysticercosis (T. solium), IgG, S 56485-6