Method Description

Trypanosoma cruzi Total Antibody:

The Wiener Chagatest ELISA recombinante v.3.0 test kit is a qualitative technique for the detection of anti-Trypanosoma cruzi antibodies. The sample is diluted in the support in which the recombinant antigen (1, 2, 13, 30, 36, and SAPA) is immobilized (3rd generation method). These antigens are obtained by DNA recombinant techniques starting from specific proteins from the epimastigote and trypomastigote stages of the T cruzi corresponding to highly conserved zones among different strains. The technology used allows us to ensure an antigenic mixture of known and permanent composition batch to batch, giving reproducible, specific, and highly sensitive results. If the sample contains specific antibodies, these will form a complex with the antigens and will remain bound to the support. The unbound fraction is eliminated by washing, after which antihuman immunoglobulin antibodies conjugated to peroxidase are added. If a reaction is produced in the first step of the process, the conjugate is bound. After a new wash, the enzymatic substrate is added. If bound conjugate is present, a light-blue color is developed. The reaction is stopped by adding sulfuric acid, and the color changes to yellow.(Package insert: Chagatest ELISA recombinante v.3.0. Wiener Laboratorios S.A.I.C.; 801146000/00)

Trypanosoma cruzi IgG:

The Chagas DetectTM Plus Rapid Test is a qualitative, membrane-based immunoassay for the detection of antibodies to Trypanosoma cruzi in human serum and whole blood matrices (venous and capillary [finger prick] whole blood). The rapid test membrane is precoated with a recombinant antigen on the test line region and utilizes a separate control to assure assay flow and performance. During testing, the sample and a proprietary blend of a stable liquid conjugate labeled with protein A are added to the sample pad. The conjugate and serum mixture migrates upward on the membrane (via capillary action) to react with recombinant T cruzi antigen on the membrane. If antibodies to the T cruzi antigen are present, a red line will appear at the test line. The red line at the control region should always appear if the assay is performed correctly. The presence of this red line verifies that proper flow has occurred and catastrophic failure of the conjugate has not occurred.(Package insert: Chagas Detect Plus Rapid Test. InBios; 01/05/2018)