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HelpTest Code CRPCR Carbapenem Resistance Genes, Molecular Detection, PCR, Rectal Swab
Ordering Guidance
This test is performed on rectal and perirectal swab specimens from patients at risk for intestinal colonization with bacteria not susceptible to carbapenem antimicrobials.
Other mechanisms of carbapenem resistance, including carbapenemases not targeted by this assay, porin mutations, and hyperexpression of drug efflux pumps, may result in carbapenem resistance. These mechanisms are not detected by this assay.
Specimen Required
Specimen Type: Rectal/perirectal swab
Supplies: Culturette (BBL Culture Swab) (T092)
Container/Tube: Culture transport swab
Specimen Volume: Entire collection
Secondary ID
620176Useful For
Detecting and differentiating the blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP gene sequences associated with carbapenem intermediate or resistant results
Aiding in infection control in the detection of gastrointestinal colonization of patients in healthcare settings with bacteria not susceptible to carbapenems using rectal or perirectal swabs
Method Name
Real-Time Polymerase Chain Reaction (RT-PCR)
Specimen Type
SwabSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Swab | Ambient | 5 days |
Reject Due To
| Gross lipemia | Reject |
| Incorrect swab used | Reject |
Reference Values
Not detected
Interpretation
A detected result is when blaKPC, blaNDM, blaVIM, blaOXA-48, or blaIMP target DNA is detected. This indicates the presence of gene sequences associated with carbapenem intermediate or resistant results.
A not detected result is when blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP target DNA is not detected.
A not detected Xpert Carba-R Assay result does not preclude the presence of other carbapenem resistance mechanisms.
Method Description
The GeneXpert Dx System automates and integrates sample purification, nucleic acid amplification, and detection of the target sequence in simple or complex specimens using a real-time polymerase chain reaction (PCR) assay. The system requires the use of single-use disposable cartridges that hold the PCR reagents and host the PCR process. Because the cartridges are self-contained, cross-contamination between samples is eliminated. The primers and probes in the Xpert Carba-R Assay detect proprietary sequences for the blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP gene sequences associated with carbapenem intermediate or resistant results in gram-negative bacteria.(Package insert: Xpert Carba-R. Cepheid; 01/2018)
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 daySpecimen Retention Time
5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CRPCR | Carbapenem Resistance Genes, PCR | 85502-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| IMPCR | IMP Resistance Gene | 85498-4 |
| VIMCR | VIM Resistance Gene | 85501-5 |
| NDMCR | NDM Resistance Gene | 73982-1 |
| KPPCR | KPC Resistance Gene | 49617-4 |
| OXACR | OXA-48-like Resistance Gene | 85503-1 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.