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Specimen Collection Guidelines

Blood Draw Laboratories at MUSC

MUSC Requisitions and Supply Requests

Lab Supply Requests

Mayo Clinic Laboratories

Test Code CMVLR Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory


Ordering Guidance


For plasma specimens, order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.



Necessary Information


Specimen source is required.



Specimen Required


Specimen Type: Lower respiratory

Source: Bronchial washing, bronchoalveolar lavage, fluid/washings from lung, sputum, tracheal secretions, tracheal aspirates

Container/Tube:

Preferred: Sterile, screwcap, 5-mL aliquot tube

Acceptable: Sterile container

Specimen Volume: 1 mL

Collection Instructions: Do not centrifuge.


Secondary ID

621770

Useful For

Rapid qualitative detection of cytomegalovirus (CMV) DNA in lower respiratory specimens

 

This test is not intended for the monitoring of CMV disease progression or response to therapy.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Specimen Type

Varies

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Lower respiratory swab
Calcium alginate-tipped swab
Wood swab
Transport swab containing gel
Feces
Paraffin blocks
Tissue specimens
Tissue biopsy
Bronchial brushings
Heat-inactivated specimens
Lower respiratory in transport media		 Reject

Reference Values

Negative

 

Reference values apply to all ages.

Interpretation

A positive result indicates the presence of cytomegalovirus (CMV) DNA in the patient specimen.

 

A negative result indicates the absence of CMV DNA in the patient specimen but does not rule out possible infection with CMV.

 

An invalid result indicates the inability to conclusively determine presence or absence of CMV DNA in the patient specimen.

Method Description

The Simplexa Congenital CMV (cytomegalovirus) Direct assay is a real-time polymerase chain reaction (PCR) system that enables the direct amplification and detection of CMV DNA from lower respiratory specimens without nucleic acid extraction. The system consists of the Simplexa Congenital CMV Direct Reaction Mix, the LIAISON MDX (with LIAISON MDX Studio Software), the direct amplification disc, and associated accessories.

 

In the Simplexa Congenital CMV Direct assay, bifunctional fluorescent probe-primers are used together with corresponding reverse primers to amplify CMV DNA. A well-conserved region of the CMV UL83 gene is targeted to identify CMV DNA. An internal control is used to detect PCR failure or inhibition.(Package insert: Simplexa Congenital CMV Direct. Diasorin; REF MOL2255. Rev. 01, 11/2022)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87496

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CMVLR Cytomegalovirus, PCR, Lower Resp 104760-4

 

Result ID Test Result Name Result LOINC Value
CMVSS Specimen Source 31208-2
621771 CMVLR, PCR 104760-4