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HelpTest Code CHAGL Trypanosoma cruzi IgG, Lateral Flow Assay, Serum
Specimen Required
Only orderable as part of a profile. For more information see CRUZI / Trypanosoma cruzi (Chagas) Antibody Panel, Serum.
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.2 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
622447Useful For
Diagnosis of chronic Trypanosoma cruzi infection (Chagas disease)
Method Name
Only orderable as part of a profile. For more information see CRUZI / Trypanosoma cruzi (Chagas) Antibody Panel, Serum.
Lateral Flow Assay (LFA)
Specimen Type
SerumSpecimen Minimum Volume
0.10 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
Only orderable as part of a profile. For more information see CRUZI / Trypanosoma cruzi (Chagas) Antibody Panel, Serum.
Negative
Reference values apply to all ages.
Interpretation
|
CHAGS result |
CHAGL result |
Interpretive comment |
|
Positive |
Positive |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by two separate methods, suggesting current or past infection. Results should be interpreted alongside clinical presentation and exposure history. |
|
Positive |
Negative |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by one of two assays. Discordant results can be resolved through submission of a new sample for testing or through additional testing at a public health laboratory. |
|
Positive |
Invalid |
Submission of a new sample is recommended to resolve discordant results. |
|
Indeterminate |
Positive |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by one of two assays. Discordant results can be resolved through submission of a new sample for testing or through additional testing at a public health laboratory. |
|
Indeterminate |
Negative |
Submission of a new sample is recommended to resolve discordant results. |
|
Indeterminate |
Invalid |
Submission of a new sample is recommended to resolve discordant results. |
|
Negative |
Positive |
Antibodies to Trypanosoma cruzi (Chagas disease) detected by one of two assays. Discordant results can be resolved through submission of a new sample for testing or through additional testing at a public health laboratory. |
|
Negative |
Negative |
No antibodies to Trypanosoma cruzi (Chagas disease) detected. False negative results may occur in patients tested within 4 weeks of infection. |
|
Negative |
Invalid |
Submission of a new sample is recommended to resolve discordant results. |
Method Description
The Chagas DetectTM Plus Rapid Test is a qualitative, membrane-based immunoassay for the detection of antibodies to Trypanosoma cruzi in human serum and whole blood matrices (venous and capillary [finger prick] whole blood). The rapid test membrane is precoated with a recombinant antigen on the test line region and utilizes a separate control to assure assay flow and performance. During testing, the sample and a proprietary blend of a stable liquid conjugate labeled with protein A are added to the sample pad. The conjugate and serum mixture migrates upward on the membrane (via capillary action) to react with recombinant T cruzi antigen on the membrane. If antibodies to the T cruzi antigen are present, a red line will appear at the test line. The red line at the control region should always appear if the assay is performed correctly. The presence of this red line verifies that proper flow has occurred and catastrophic failure of the conjugate has not occurred.(Package insert: Chagas Detect Plus Rapid Test. InBios; 01/05/2018)
Day(s) Performed
Monday
Report Available
Same day/1 to 8 daysSpecimen Retention Time
2 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86753
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CHAGL | T. cruzi IgG, LFA, S | 87994-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CHAGL | T. cruzi IgG, LFA, S | 87994-0 |