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Test Code BTBAS Butabarbital, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Draw blood immediately before the next scheduled dose.

2. Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Secondary ID

622885

Useful For

Monitoring of butabarbital therapy

Method Name

Gas Chromatography Mass Spectrometry (GC-MS)

Specimen Type

Serum Red

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Therapeutic range:

2.0-3.0 mcg/mL Hypnosis

25 mcg/mL Sedation/Sleep

>30 mcg/mL Coma

Toxic concentration: >50 mcg/mL

 

Cutoff concentrations by gas chromatography mass spectrometry:

Butabarbital: 0.5 ng/mL

Interpretation

Butabarbital concentrations between 2.0 and 3.0 mcg/mL have been used for hypnosis/sedation with concentrations up to 25 mcg/mL used for sleep. Toxic concentrations have been reported as greater than 50 mcg/mL.

Method Description

Barbiturates are extracted from serum using solid-phase extraction techniques. The serum is buffered and eluted with organic solvent. The organic phase is dried, reconstituted, and the analysis performed by gas chromatography mass spectrometry using selected ion monitoring. The assay utilizes deuterated barbiturates as internal standards.(Unpublished Mayo method)

Day(s) Performed

Thursday

Report Available

3 to 9 days

Specimen Retention Time

2 weeks

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BTBAS Butabarbital, S 16236-2

 

Result ID Test Result Name Result LOINC Value
622885 Butabarbital, S 16236-2